Northeastern University – Holographic Imaging Cytometry Program of Excellence
The joint program of excellence between Northeastern University and PHI began in late 2014. Since then HoloMonitor technology has rapidly established itself as a technology of choice for label-free kinetic cellular analysis. The program covers several activities including:
- Establishing a network of collaborators utilizing Holographic Imaging Cytometry.
- Developing novel applications supporting research interests of the Northeastern faculty and students.
- Presenting results at scientific meetings.
- Organizing an annual technology symposium and hands-on laboratory courses.
Peer Reviewed Articles and Book Chapters
Applications of quantitative time lapse holographic imaging to the development of complex pharmaceutical nano formulationsSPIE BiOS Conference Proceedings (2018)Read more
We rely on in vitro cellular cultures to evaluate the effects of the components of multifunctional nano-based formulations under development. We employ an incubator-adapted, label-free holographic imaging cytometer HoloMonitor M4® (Phase Holographic Imaging, Lund, Sweden) to obtain multi-day time-lapse sequences at 5- minute intervals. An automated stage allows hand-free acquisition of multiple fields of view.
Applications of Label-free, Quantitative Phase Holographic Imaging Cytometry to the Development of Multi-specific Nanoscale Pharmaceutical FormulationsCytometry Part A 2017 (2017)Read more
A review of HoloMonitor applications: tracking of Giant HeLa cells, which may be undergoing neosis tracking the effects of cell cycle related toxic agents on cell lines; using MicroRNAs to reverse the polarization state in macrophages to induce tumor cell killing development of liposomal nanoformulations to overcome multi-drug resistance in ovarian cancer cells and development of dual sensitive micelles to specifically target matrix metalloproteinase 2
Reversal of Chemoresistance in Ovarian Cancer by Co-delivery of a p-glycoprotein Inhibitor and Paclitaxel in a Liposomal PlatformMolecular Cancer Therapeutics (2016)Read more
The authors created a nanoparticle formulation against chemotherapy resistant ovarian cancer, loaded with the commonly used cancer drug paclitaxel together with a drug that breaks down the resistance mechanism of cancer cells. Using HoloMonitor, the scientists were able to show that paclitaxel-resistant ovarian cancer cells stopped multiplying when treated with the nanoparticles.
Mixed Nanosized Polymeric Micelles as Promoter of Doxorubicin and Mirna-34a Co-delivery Triggered by Dual Stimuli in Tumor TissueSmall (2016)Read more
Methods for producing 4D plot were developed. Images from the time course were exported to ImageJ and treated as an image stack, creating a volume rendering of the cellular motion over time of analysis. The brightness threshold was adjusted to view all cells within the imaging area or only the thicker cells, a feature that allows identification of mitosis, apoptosis, and transition between mesenchymal and amoeboid cellular morphologies
Enhanced Cytotoxicity of Folic Acid-targeted Liposomes Co-loaded With C6 Ceramide and Doxorubicin: in Vitro Evaluation on HeLa, A2780-ADR and H69-AR CellsMolecular pharmaceutics (2015)Read more
Using holomonitor M4, it was found that FA-(C6+Dox)-LP treated hela cells underwent rapid progression to apoptosis (cell suicide) after 21 hours, as evidenced by a drastic drop in cell area after loss of cell membrane integrity.
Macrophage Polarization and the Effect of Microrna-155 Administered in Water-in-oil-in-water Multiple Emulsion FormulationsJournal of Clinical & Cellular Immunology (2015)Read more
Holographic assessment using Holomonitor M4 of the co-cultured cells in real-time showed differences in motility and morphology of macrophages with mir-155 ME treated cells showing greater cellular interaction between the two phenotypes.
Conference Papers and Posters
Comparison of the effects of pharmaceutical compounds on tumor cells in 2D and 3D in vitro models using label-free, quantitative 4 dimensional holographic imagingAACR-NCI-EORTC Inter. Conf. on Molecular Targets and Cancer Therapeutics 2015 (2018)
Development of a 4-Sample Version of the Kolmogorov-Smirnov Test for Evaluating the Temporal Physiology of Cells Treated with test Compounds in a Label-Free, High Content, Platform for Quantitative Analysis of Adherent Cell-Culture ModelsAACR Annual Meeting (2017)4-dimensional plots of HoloMonitor time-lapse data were developed to assist in the visualization of the effects of pharmaceutical compounds under development.
A Label-Free, High Content, Moderate Throughput Analytical Platform for Quantitative Kinetic Analysis of Cell Behavior upon Drug Activation Based on the Kolmogorov-Smirnov Test3rd Conference on Impedance-Based Cellular Assays (2016)“Our objective is to develop multi-functional nanotechnology-based antitumor drug delivery systems for improving efficacy of treatments and reducing undesirable side effects. The essential part of this process is the development of un-biased quantitative analytical techniques. We are reporting a successful validation of a very high content, medium throughput system in multi-well plates, employing the newly developed holographic imaging cytometer HoloMonitor® M4 for label-free time-lapse cellular analysis.”
Characterization of macrophage behavior by 4-dimensional label free quantitative holographic imagingAACR Annual Meeting (2015)
Four dimensional quantitative label-free holographic imaging of the cell cycle in tumor cell linesAACR Annual Meeting (2015)
Department of Pharmaceutical Sciences
School of Pharmacy
Bouvé College of Health Sciences
140 The Fenway, Room 228/229
60 Huntington Avenue
Boston, Massachusetts 02115