Time-lapse Movies

HoloMonitor Time-lapse Movies

This is a selection of representative images from various experiments using the HoloMonitor® system. All applications using HoloMonitor are label-free; colors are added in the software. You are welcome to contact us at info@phiab.se in case you wish to use or refer to images generated by HoloMonitor.

MCF-10A cells captured in their normal culture vessel without using labels or stains. The cells were imaged with the HoloMonitor M3 during 16 hours, 5 minutes between image frames.

Dividing L929 mouse fibroblast cells. One image frame every second minute was recoded. No stains or labels were applied.

Apoptotic L929 cells, treated with etoposide. The time-lapse video was recoded during 69 hours, 3 minutes between image frames.

Gap closure over time. Untreated cells are used and the gap is created using the ibidi® Culture-Insert 2 Well.

Asymmetrical cell division of JIMT-1 cells. During the video, spanning over 72 hours, one cell divides into three daughter cells (seen on top of the cell cluster in the upper right portion of the image). One of the daughter cell dies, and two cells merge. The height of the cells is color coded by the vertical look up bar on the left of the image.

An etoposide-treated DU145 prostate cancer cell exploding into a cascade of apoptotic bodies. The time-lapse was recorded during 61 hours, one image every 4 minutes. The optical thickness is color coded. With increasing thickness, color changes from gray to yellow, red, purple and finally black.

Multiple L929 cells divisions, recorded by the HoloMonitor M3. Notice how cells interact with neighboring cells before they divide.

Cell within a cell. The JIMT-1 cells was imaged by the HoloMonitor M3.

Reluctant cell division. The cell in the center of video curls up several time to divide. However, it changes its mind. It's not until after it has interacted with the neighboring cells that it decides to finally divide. The time-lapse micro­scopy video was recorded by the Holo­Monitor M3.

HUVEC cells, imaged by the HoloMonitor M3. One image frame was recorded every 5 minutes.

Chemotaxis time-lapse of a gel cell culture, created using HoloMonitor M3. Images were acquired every 5 minutes for 16 hours. The HT-1080 cells were grown in a gel matrix on a μ-Slide Chemotaxis 3D slide from IBIDI. Bovine serum was used to stimulate cell motion.

Non-mitotic cell division (cell budding) of a multi-nuclei L929 cell, created using HoloMonitor M4. Non-mitotic cell division have been reported but never filmed before.