Below you will find summaries and links to peer reviewed articles and other publications and conference papers discussing HoloMonitor applications and PHI's core technology – holographic and quantitative phase imaging (QPI) microscopy.

Highlighted Articles

  • Evaluation of Holographic Imaging Cytometer HoloMonitor M4® Motility Applications
    Y. Zhang and R. L. Judson
    Cytometry Part A (2018)

    The HoloMonitor software modules for cell tracking and wound healing analysis were evaluated and compared to the more conventional methods transwell migration and transwell invasion. Both HoloMonitor modules were found to be well-correlated with established standards, yielded reproducible results, and at the same time offered distinct advantages. The wound healing assay was the most tractable and automated method with good reproducibility, while the cell tracking module enabled identification of hypermobile subpopulations.

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  • Bi-allelic Loss of CDKN2A Initiates Melanoma Invasion via BRN2 Activation
    H. Zeng et al.
    Cancer Cell (2018)

    Utilizing precision genetic engineering and PHI’s HoloMonitor technology, scientists at University of California, San Francisco, have for the first time been able to monitor and map how mutations break down the genetic protection against skin cancer, allowing harmless moles to transform into invasive skin cancer.

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  • Combined activation of MAP kinase pathway and β-catenin signaling cause deep penetrating nevi
    I. Yeh et al.
    Nature Communications (2017)

    HoloMonitor was used to measure cell volume. Together with other methods the results identify DPN (deep penetrating nevus) as an intermediate melanocytic neoplasm, with a progression stage positioned between benign nevus and DPN-like melanoma.

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  • Loss of Erα Induces Amoeboid-like Migration of Breast Cancer Cells by Downregulating Vinculin
    Y. Gao et al.
    Nature Communications (2017)

    The authors tracked and analyzed the migration of different MCF-7 cell populations in a 3D matrix. In addition to measuring the cell migration speed with HoloMonitor, the following five phenotypes were observed: ‘stable adhesion’, ‘unstable lamellipodia’, ‘bleb’, ‘stable bleb’ and ‘unstable pseudopod’.

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  • Reversal of Chemoresistance in Ovarian Cancer by Co-delivery of a p-glycoprotein Inhibitor and Paclitaxel in a Liposomal Platform
    Y. Zhang et al.
    Molecular Cancer Therapeutics (2016)

    The authors created a nanoparticle formulation against chemotherapy resistant ovarian cancer, loaded with the commonly used cancer drug paclitaxel together with a drug that breaks down the resistance mechanism of cancer cells. Using HoloMonitor, the scientists were able to show that paclitaxel-resistant ovarian cancer cells stopped multiplying when treated with the nanoparticles.

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Peer Reviewed Articles and Book Chapters

Conference Papers and Posters

  • Comparison of the effects of pharmaceutical compounds on tumor cells in 2D and 3D in vitro models using label-free, quantitative 4 dimensional holo­graphic imaging
    Ed Luther, Giuseppina Salzano, Shravan Kumar Sriraman, Daniel Costa and Vladimir P. Torchilin
    AACR-NCI-EORTC Inter. Conf. on Molecular Targets and Cancer Therapeutics 2015 (2018)
  • Development of a 4-Sample Version of the Kolmogorov-Smirnov Test for Evaluating the Temporal Physiology of Cells Treated with test Compounds in a Label-Free, High Content, Platform for Quantitative Analysis of Adherent Cell-Culture Models
    Ed Luther, Livia Mendes, Jiayi Pan, Daniel Costa, Elena Holden and Vladimir Torchilin
    AACR Annual Meeting (2017)
    4-dimensional plots of HoloMonitor time-lapse data were developed to assist in the visualization of the effects of pharmaceutical compounds under development.
  • Cystatin C Decreases Proliferation of Melanoma Cells by Affecting Mitosis Length
    Hanna Wallin, Kersti Alm and Magnus Abrahamson
    AACR Annual Meeting (2017)
    Analysis using HoloMonitor revealed qualitative differences in the normal behavior of the A375 cells under study as a result of cystatin C addition. The motility of the cells was significantly affected. The mitosis phase was considerably prolonged in cells grown in cystatin C containing medium, indicating that the proliferation-inhibiting effect of the protease inhibitor may be through its direct action on proteolytic processes in the mitosis phase.
  • Transcription Regulation of Chemokine Receptor CXCR4 by Nuclear Respiratory Factor 1 (NRF1) Controls Estrogen-Induced Malignant Transformation of Breast Epithelial Cells to Breast Cancer Stem Cells
    J. K. Das and D. Roy
    AACR Annual Meeting (2016)
    Chemokine receptor CXCR4 is involved in the maintenance of stemness property of stem cells, aging and metastasis of cancer. NRF1 is one of the major transcription factors that controls transcription of CXCR4. In this study, the authors have investigated whether transcription regulation of CXCR4 by NRF1 controls estrogen-induced malignant transformation of breast epithelial cells to breast cancer stem cells.
  • A Label-Free, High Content, Moderate Throughput Analytical Platform for Quantitative Kinetic Analysis of Cell Behavior upon Drug Activation Based on the Kolmogorov-Smirnov Test
    Ed Luther, Livia Mendes, Jiayi Pan, Daniel Costa, Elena Holden and Vladimir Torchilin
    3rd Conference on Impedance-Based Cellular Assays (2016)
    “Our objective is to develop multi-functional nanotechnology-based antitumor drug delivery systems for improving efficacy of treatments and reducing undesirable side effects. The essential part of this process is the development of un-biased quantitative analytical techniques. We are reporting a successful validation of a very high content, medium throughput system in multi-well plates, employing the newly developed holographic imaging cytometer HoloMonitor® M4 for label-free time-lapse cellular analysis.”

    View poster

  • Impedance-Based Analysis and Holographic Phase Imaging of The GNRH-Iii-Based Drug-Targeting In Melanoma Cells
    E. Lajkó, S. Spring, O. Láng, S. Ingebrandt, G. Mező and L. Kőhidai
    3rd Conference on Impedance-Based Cellular Assays (2016)

    HoloMonitor M4 was used for the morphometry and migration studies.

    View paper

  • Characterization of macrophage behavior by 4-dimensional label free quantitative holographic imaging
    Ed Luther, Meghna Talekar, Adwait Oka, Qijun Ouyang, Mansoor Amiji.
    AACR Annual Meeting (2015)
  • Four dimensional quantitative label-free holographic imaging of the cell cycle in tumor cell lines
    Ed Luther, Jeffrey Agar and Mansoor Amiji
    AACR Annual Meeting (2015)

Doctoral Theses

  • Characterization of the Novel Pericyte Receptors S1PR3 and PTGER2
    Faculty of Biosciences, Heidelberg University
    Tina Heumann (2017)
    Morphological changes were evaluated by comparing time-plots of cell volume and thickness, created by HoloMonitor M4.
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  • Proteins, Polymers and Living Cells on Titanium Oxide-based Nano structured Coatings: Exploitation of Label-free Optical Sensing Methods
    Institute of Technical Physics and Material Science, Center for Energy Research, Hungarian Academy of Sciences
    Judit Nádor (2016)

    The HoloMonitor M4 was used to monitor living cells (human embryonic kidney cells and preosteoblasts) on nanostructured coatings in a novel cuvette configuration.

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  • Aquaporins in Infection and Inflammation
    Clinical and Experimental Medicine, Linköping University
    Angelika Holm (2016)
    By recording time-lapse videos directly within the incubator using HoloMonitor M4, volume changes of bacteria infected cells was studied.
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  • Novel Imaging Technology and Tools for Biomarker Detection in Cancer
    Health and Society, Malmö University
    Zahra El-Schich (2016)
    To analyze morphological changes of death cells, HoloMonitor was used. Digital holographic microscopy is an approach for label-free non-invasive 3D imaging of cultured cells. We have analyzed cell death of adherent cancer cells using HoloMonitor and developed it to analyze suspension cells by combining this technique with antibody based micro­assays. HoloMonitor can be used for cell-death induced cell analysis of both adherent cells and suspension cells.
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  • Combination of Unsaturated Fatty Acids and Ionizing Radiation on Human Glioma Cells: Cellular, Biochemical and Gene Expression Analysis
    Department of Pharmaceutical Technology, University of Szeged
    Otilia Antal (2016)
    HoloMonitor M3 was used to assess the increased death of glioma (brain tumor) cells when combining irradiation with various unsaturated fatty acid treatments.
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  • Modulation of Tight Junctions by Peptides to Increase Drug Penetration Across Biological Barriers
    Department of Pharmaceutical Technology, University of Szeged
    Alexandra Bocsik (2016)
    Morphological changes in peptide treated Caco-2 epithelial cells were analyzed using HoloMonitor technology.
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  • Protection of the Blood-Brain Barrier under Pathological Conditions
    University of Szeged, Faculty of Medicine and Biological Research Centre Hungarian Academy of Sciences, Szeged
    Andrea Tóth (2014)

    Endothelial cells were cultured on collagen coated culture dishes with borosilicate glass bottom. HoloMonitor was used to analyze cell morphological changes before and during treatments.

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  • Nanowires in Cell Biology – Exploring Interactions and Applications
    Division of Solid State Physics, Lund University
    Henrik Persson (2014)
    Using HoloMonitor M4 this thesis explores the interactions between cells and nanowires, to increase our under­standing of how cells are affected and how they can be manipulated by these one-dimensional, semiconductor, crystals.
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  • Design of Reconfigurable Hardware Architectures for Real-Time Applications
    Department of Electrical and Information Technology, Lund University
    T. Lenart (2008)