Below you will find summaries and links to peer reviewed articles and other publications and conference papers discussing HoloMonitor applications and PHI's core technology – holographic and quantitative phase imaging (QPI) microscopy.

Peer Reviewed Articles and Book Chapters

  • Evaluation of Holographic Imaging Cytometer HoloMonitor M4® Motility Applications
    Y. Zhang and R. L. Judson
    Cytometry Part A (2018)

    The HoloMonitor software modules for cell tracking and wound healing analysis were evaluated and compared to the more conventional methods transwell migration and transwell invasion. Both HoloMonitor modules were found to be well-correlated with established standards, yielded reproducible results, and at the same time offered distinct advantages. The wound healing assay was the most tractable and automated method with good reproducibility, while the cell tracking module enabled identification of hypermobile subpopulations.

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  • TRPM2 Modulates Neutrophil Attraction to Murine Tumor Cells by Regulating CXCL2 Expression
    M Gershkovitz et al
    Cancer Immunology, Immunotherapy (2018)

    The tumor microenvironment in vivo is not limited to the tumor cells themselves but also includes many kinds of immune cells. In this study the researchers explored cytotoxic effects of neutrophils in a co-culture with murine tumor cells. To exert their cell killing effect the neutrophils must reach the target cells, i.e. tumor cells. Using the HoloMonitor cell motility and migration assays the scientists could explore how the ability of the neutrophils to approach the cancer cells was affected during various conditions. Their findings are also supported and visualized in an associated time-lapse movie.

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  • Translational Medicine in Pulmonary-Renal Crosstalk: Therapeutic Targeting of p-Cresyl Sulfate Triggered Nonspecific ROS and Chemoattractants in Dyspneic Patients with Uremic Lung Injury
    J-F Chang et al
    J. Clin. Med. (2018)

    Chronic kidney disease causes kidney failure, but there are also many side effects, e.g. respiratory distress called uremic lung injury. P-Cresyl sulfate (PCS) is produced when proteins ferment in intestinal bacteria, and accumulates in patients with chronic kidney disease. The authors found an effect of PCS on lung cancer cells as measured by cell count using HoloMonitor. The higher the concentration the more effect, until cell death was initiated. They tried to inhibit the effect of PCS with several different substances and used cell count here as well.

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  • Imidazole Compounds for Protecting Choroidal Endothelial Cells from Complement Injury
    Zeng S et al.
    Scientific Reports (2018)

    AMD (age-related macular degeneration) is a common and serious eye disease, affecting mostly elderly people. The authors found two drug candidates that seemed to protect endothelial cells from the kind of complement-induced injury and cell death seen in AMD. Using HoloMonitor M4 is was shown that drug pretreatment reduced the cell surface roughness, thus indicating improved cell health.

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  • Hexagonal Boron Nitride Functionalized with Au Nanoparticles – Properties and Potential Biological Applications
    M. Jedrzejczak-Silicka et al.
    Nanomaterials (2018)

    Due to its chemical properties hexagonal boron nitride with gold nanoparticles may be an interesting material for cancer drug delivery etc. In this study, potential biomedical applications and cytotoxicity of the material was evaluated. HoloMonitor M4 was used as a tool to determine the effects on cell proliferation and cell morphology over time, after exposue to the material.

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  • General Cytotoxicity and Its Application in Nanomaterial Analysis
    M. Jedrzejczak-Silicka and E. Mijowska
    IntechOpen (2018)

    Review describing and comparing various assays used to study biocompatibility and cytotoxicity of nanomaterials. Holographic phase imaging is pointed out as an excellent tool for cell morphometric characterisation and cell migration studies, and the authors conclude that the interest in the use of DH microscopy in research is constantly increasing.

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  • Bi-allelic Loss of CDKN2A Initiates Melanoma Invasion via BRN2 Activation
    Hanlin Zeng et al.
    Cancer Cell (2018)

    Utilizing precision genetic engineering and PHI’s HoloMonitor technology, scientists at University of California, San Francisco, have for the first time been able to monitor and map how mutations break down the genetic protection against skin cancer, allowing harmless moles to transform into invasive skin cancer.

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  • CAT3, a prodrug of 13a(S)-3-hydroxyl-6,7-dimethoxyphenanthro[9,10-b]-indolizidine, circumvents temozolomide-resistant glioblastoma via the Hedgehog signaling pathway, independently of O6-methylguanine DNA methyltransferase expression
    M. Ji et al.
    OncoTargets and Therapy (2018)

    The results indicate that CAT3 is a potential therapeutic agent for TMZ-resistant GBM (glioblastoma multiforme). HoloMonitor M4 was used to directly monitor and calculate the cell number.

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  • Quantitative Phase Imaging for Label-Free Analysis of Cancer Cells – Focus on Digital Holographic Microscopy
    Z. El-Schich, A Leida Mölder and A Gjörloff Wingren
    Appl Sci (2018)

    Review providing a current insight into QPI (quantitative phase imaging) applied to cancer research. The authors conclude that QPI is an ideal method for studying live cell dynamics by providing data from noninvasive monitoring over arbitrary time scales. The effect of drugs on migration, proliferation, and apoptosis of cancer cells are emerging fields suitable for QPI analysis. The authors also point out that need for QPI applications in clinical cancer diagnostics and treatments is emerging.

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  • Quantitative evaluation of morphological changes in activated platelets in vitro using digital holographic microscopy
    Y Kitamura et al.
    Micron (2018)

    The feasibility of DHM (Digital Holographic Microscopy) in the quantitative evaluation of morphological changes in activated platelets was evaluated. Data shown implies that DHM using HoloMonitor could be a promissing method for quantitative examination of morphological changes in platelets in vitro. The publication also includes a comparision of the performance of different techniques for this purpose.

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  • The impact of circulating preeclampsia-associated extracellular vesicles on the migratory activity and phenotype of THP-1 monocytic cells
    Kovács ÁF, Láng O, Turiák L, Ács A, Kőhidai L, Fekete N, Alasztics B, Mészáros T, Buzás E I and Rigó J Jr. Pállinger
    Scientific Reports (2018)

    The study suggests that circulating extracellular vesicles (EVs) activate THP-1 monocytes, induce intra-monocytic TNF expression, as well as an altered chemotaxis and cell migration pattern. This is the first study to investigate in preeclampsia the effects of circulating EVs on monocyte chemotaxis, cell adhesion and migration in vitro.

    The HoloMonitor M4 was used for cell tracking of adherent cells. In the present experiments the chemokinetic effects were evaluated by measurement and calculation of migration and motility.

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  • Applications of quantitative time lapse holographic imaging to the development of complex pharmaceutical nano formulations
    E Luther, L Mendes, J Pan, D Costa, C Sarasozen and V Torchilin
    SPIE BiOS Conference Proceedings (2018)

    We rely on in vitro cellular cultures to evaluate the effects of the components of multifunctional nano-based formulations under development. We employ an incubator-adapted, label-free holographic imaging cytometer HoloMonitor M4® (Phase Holographic Imaging, Lund, Sweden) to obtain multi-day time-lapse sequences at 5- minute intervals. An automated stage allows hand-free acquisition of multiple fields of view.

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  • Quantification of mammalian tumor cell state plasticity with digital holographic cytometry
    M Hejna, A Jorapur, Y Zhang, J S Song and R L Judson
    SPIE Conference Proceedings (2018)

    Working with a HoloMonitor M4 digital holographic cytometry platform, we have established a machine learning-based pipeline for high accuracy and label-free classification of adherent cells.

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  • Label-free optical biosensor for real-time monitoring the cytotoxicity of xenobiotics: A proof of principle study on glyphosate
    E Farkasa, A Szekacsc, B Kovacsa, M Olahc, R Horvatha and I Szekacs
    Journal of Hazardous Materials (2018)

    In the present work, the herbicide product Roundup Classic, its formulant (POEA) and its active ingredient (glyphosate) were studied on a mammalian osteoblastic cell line (MC3T3-E1) model using the optical biosensor Epic BT. Cell viability was monitored by HoloMonitor M4 by analyzing three-dimensional morphological structures of the cells.

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  • The evolution of phase holographic imaging from a research idea to publicly traded company
    P Egelberg
    SPIE BiOS Company Proceedings (2018)

    It is discussed how relevant biological information can be extracted from a sequence of quantitative phase images, with negligible user assistance and parameter tweaking, to simultaneously provide cell culture characteristics such as cell growth rate, viability, division rate, mitosis duration, phagocytosis rate, migration, motility and cell-cell adherence without requiring any artificial cell manipulation.

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  • Contribution of TMEM16F to pyroptotic cell death
    J Ousingsawat, P Wanitchakool, R Schreiber and K Kunzelmann
    Cell Death and Disease (2018)

    The role of TMEM6F in pyroptosis was investigated and the data suggests that TMEM16F has a significant impact on pyroptotic cell lysis and subsequent phagocytosis by neutrofils. HoloMonito was used to analyse cell volume changes.

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  • A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells
    J Huang, P Guo and Marsha A. Moses
    JoVe (video journal) (2018)

    The video and publication demonstrate how HoloMonitor is used to conduct time-lapse and labeling-free characterizations of angiogenic and non-angiogenic human osteosarcoma KHOS cells. A panel of cellular parameters, including cell morphology, proliferation, and motility, were quantitatively measured and analyzed using HoloMonitor.

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  • β-Nitrostyrene 1 derivatives attenuate LPS-mediated acute lung injury via the 2 inhibition of neutrophil-platelet interactions and NET release
    Yao-Wen Chang et al
    American Journal of Physiology-Lung Cellular and Molecular Physiology (2018)

    Time-lapse videos and cell morphology images were recorded with a HoloMonitor M4 to assess the effects of the compound on neutrophil-platelet interaction and activation

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  • Kynurenic acid and its derivatives are able to modulate the adhesion and locomotion of brain endothelial cells
    E Lajkó, B Tuka, F Fülöp, I Krizbai, J Toldi and K Magyar
    Journal of Neural Transmission (2018)

    Holomonitor M4 was used to analyse both chemokinetic responses and morphometry. The results obtained on endothelial responses
    and its amide derivatives.

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  • iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Management
    S-X Cheng et al
    Neurotherapeutics (2017)

    Targeted temperature management (TTM) modulation on traumatic brain injury (TBI). HoloMonitor was used to analyze cell area and cell volume after exposure of cells to hypothermia or normothermia.

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  • The role of Vimentin in Regulating Cell Invasive Migration in Dense Cultures of Breast Carcinoma Cells
    Y. Messica
    Nano Letters (2017)
  • Tau Phosphorylation and Amyloid-β Deposition in the Presence of Formaldehyde
    J. Lu, R. He
    Formaldehyde and Cognition (2017)

    Mechanisms related to Alzheimer’s disease (AD). Formaldehyde is shown to be a strong trigger of Aβ deposition and Tau phosphorylation, so that the dysmetabolism of endogenous formaldehyde should occur in the early stage of AD as formaldehyde disturbs nervous system in different pathways and in diverse manners.

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  • NatD promotes lung cancer progression by preventing histone H4 serine phosphorylation to activate Slug expression
    J. Ju et al
    Nature Communications (2017)

    The authors report that NatD (N-α-acetyltransferase D) promotes the migratory and invasive capabilities of lung cancer cells in vitro and in vivo. On the other hand, depletion of NatD suppresses the epithelial-to-mesenchymal transition (EMT) of lung cancer cells. These findings indicate that NatD is a crucial epigenetic modulator of cell invasion during lung cancer progression.

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  • Zinc enhances the cellular energy supply to improve cell motility and restore impaired energetic metabolism in a toxic environment induced by OTA
    X. Yang
    Scientific Reports (2017)

    Investigating the function of zinc in stimulated cell motility and motility speed in normal cell lines. Afterwards, energy metabolism pathways and impaired energetic metabolism models were introduced to show the function of zinc in the cellular energy supply.

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  • Influence of salinomycin treatment on division and movement of individual cancer cells cultured in normoxia or hypoxia evaluated with time-lapse digital holographic microscopy
    S. Kamlund, D. Strand, B. Janicke, K. Alm, S Oredsson
    Cell Cycle (2017)

    Digital holographic microscopy was used for longitudinal cell tracking of individual cells. Various parameters were investigated and the results suggest that EMT (epithelial to mesenchymal transision)  and MET (mesenchymal to epithelial transission) can be investigated using this method.

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  • A Biophysical Approach to Quantify Skeletal Stem Cells Trans-differentiation as a Model for the Study of Osteoporosis
    L. Petecchia, F. Viti, F. Sbrana, M. Vassalli and P. Gavazzo
    Biophysical Chemistry (2017)

    The authors wrote their own software for multi-parameter morphology analysis. Digital Holography allowed to extract a set of quantitative indicators associated with the specific biological state and, among all possible parameters, the average thickness, T, and the roughness kurtosis, RK, allowed to produce a scatter plot in which full osteoblasts, full adipocytes and undifferentiated hBM-MSC lie in well separated regions of the space. Noteworthy, this T-RK graph was successfully extended to all conditions and provided a clear and prompt visualization of the cellular commitment in terms of position in the T-RK plane.

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  • Interleukin-6 blockade attenuates lung cancer tissue construction integrated by cancer stem cells
    H. Ogawa, M. Koyanagi-Aoi, K. Otani, Y. Zen, Y. Maniwa, T. Aoi
    Scientific Reports (2017)

    The authors established technologies to generate lung CSC (cancer stem cell) -like cells from human lung cancer cell line A549 by introducing OCT3/4, SOX2 and KLF4, and to construct “lung cancer organoids” in vitro that mimicked human lung cancer tissues. Through the use of these technologies and the evaluation of clinical samples, interleukin-6 was identified as a novel potential therapeutic target for lung cancer stem cells.

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  • Combined activation of MAP kinase pathway and β-catenin signaling cause deep penetrating nevi
    I. Yeh et al.
    Nature Communications (2017)

    HoloMonitor was used to measure cell volume. Together with other methods the results identify DPN (deep penetrating nevus) as an intermediate melanocytic neoplasm, with a progression stage positioned between benign nevus and DPN-like melanoma.

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  • Biophysical characterization of nanostructured TiO2 as a good substrate for hBM-MSC adhesion, growth and differentiation
    L. Petecchia, C. Usai, M. Vassalli and P. Gavazzo
    Experimental Cell Research (2017)

    The aim of the study was to evaluate the effectiveness of a nano-patterned titanium surface in sustaining hBM-MSC adhesion, growth and differentiation.

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  • High accuracy label-free classification of single-cell kinetic states from holographic cytometry of human melanoma cells
    M. Hejna et al.
    Scientific Reports (2017)

    The authors used machine learning to develop a method for robust and kinetic label-free classification of single adherent cells info functional states.

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  • Dynamics of Morphological Changes of Adherent Cultured Cells at a Range of Low Temperatures Near Physiological Temperature (Time-lapse Imaging by Digital Holographic Microscopy)
    Hiroshi Ishiguro, Makoto Uemura and Kouhei Nagasaki
    Cryobiology and Cryotechnology (2017)

    This study investigated the dynamics of morphological changes, including mobility of adherent cultured cells, under low-temperature stress near physiological temperature, using time-lapse three-dimensional imaging with digital holographic microscopy (DHM).

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  • Biophysical Characterization of Nanostructured TiO2 as a Good Substrate for hBM-MSC Adhesion, Growth and Differentiation
    L. Petecchia, C. Usai, M. Vassalli and P. Gavazzo
    Experimental Cell Research (2017)

    The aim of the study was to evaluate the effectiveness of a nano-patterned titanium surface in sustaining hBM-MSC adhesion, growth and differentiation.

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  • CFTR supports cell death through ROS-dependent activation of TMEM16F (anoctamin 6)
    Y. Messica
    Eur J Physiol (2017)

    Mechanism in cystic fibrosis; role of induced cell death by reactive oxygen species (ROS) was investigated. Cell volume measurements using HoloMonitor was used to assess cell shrinkage during cell death.

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  • Standardized Polyalthia Longifolia Leaf Extract (Plme) Inhibits Cell Proliferation and Promotes Apoptosis: the Anti-cancer Study With Various Microscopy Methods
    Soundararajan Vijayarathnaa, Yeng Chenb, Jagat R. Kanwarc and Sreenivasan Sasidharana
    Biomedicine & Pharmacotherapy (2017)

    In this study, the apoptogenic property and the cytotoxic effect of P. longifolia leaf methanolic extract (PLME) against the human cervical carcinoma cells (HeLa) was studied using light microscope (LM), digital holographic digital microscopy (DHM), scanning electron microscope (SEM) and transmission electron microscope (TEM).

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  • Synthesis, characterization and in vitro and in vivo photodynamic activities of a gallium(iii) tris(ethoxycarbonyl)corrole
    Zhao Zhang, Hua-Hua Wang, Hua-Jun Yu, Yu-Zhen Xiong, Hai-Tao Zhang, Liang-Nian Jid and Hai-Yang Liu
    Dalton Transactions 2017 (2017)

    After illumination and treatment with 1-Ga, A549 cells exhibited characteristics typical of apoptosis, spherical shape of the cells, chromatin condensation, and nuclear membrane irregularity. After incubation for 1 h, the authors found that the cells started to contract, the area decreased and the thickness increased.

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  • Hypoxia-inducible Factor-1 Alpha Is Involved in Rip-induced Necroptosis Caused by in Vitro and in Vivo Ischemic Brain Injury
    Xiao-Sa Yang, Tai-Long Yi, Sai Zhang, Zhong-Wei Xu, Ze-Qi Yu, Hong-Tao Sun, Cheng Yang, Yue Tu & Shi-Xiang Cheng
    Scientific reports (2017)

    These findings indicate that necroptosis contributes to ischemic brain injury induced by OGD and MCAO and implicate HIF-1α, RIP1, RIP3, and MLKL in necroptosis.

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  • Accumulation of Simulated Pathological Level of Formaldehyde Decreases Cell Viability and Adhesive Morphology in Neuronal Cells
    Wang Jing, Zhou Jun, MO Wei-Chuan, He Ying-Ge, Wei Yan, He Rong-Qiao and Yi Fa-Ping
    Progress in Biochemistry and Biophysics (2017)

    Chronic formaldehyde (FA) exposure leads to impairment of human memory. Using HoloMonitor Wang Jing and coworkers illustrate how FA exposure influence the cell morphology in terms of thickness, area and volume.

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  • Biophysical characterization of nanostructured TiO2 as a good substrate for hBM-MSC adhesion, growth and differentiation
    L. Petecchia, C. Usai, M. Vassalli, and P.Gavazzo
    Experimental Cell Research (2017)

    Evaluation of the effectiveness of a nano-patterned titanium surface in sustaining hBM-MSC adhesion, growth and differentiation by means of a panel of biophysical tools: morphometry, electrophysiology, intracellular calcium measurements and immunocytochemistry. The results substantiate the idea that this micro-textured titanium dioxide is a good surface for growth and differentiation of hBM-MSC and it exhibits a stimulating action mainly in the initial period of differentiation. HoloMonitor was used for analysis of various morphological parameters.

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  • Ouabain suppresses the growth and migration abilities of glioma U‑87MG cells through inhibiting the Akt/mTOR signaling pathway and downregulating the expression of HIF‑1α
    Xiao‑Sa Yang et al
    Molecular Medicine Reports (2017)

    The present study revealed that ouabain intervention inhibited U‑87MG cell viability and cell motility in a dose‑dependent manner, suggesting that ouabain promoted the apoptosis of U87‑MG cells. HoloMonitor was used to analyse cell motility, cell count and to study the 3D cell structure.

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  • Mannich Curcuminoids as Potent Anticancer Agents
    M. Gyuris, L. Hackler, L. Nagy, R. Alföldi, E. Rédei, A. Marton, T. Vellai, N. Faragó, B. Ózsvári, A. Hetényi, G. Tóth, P. Sipos, I. Kanizsai and L. Puskás
    Archiv der Pharmazie (2017)

    Structure–activity relationship (SAR) studies were performed by applying viability assays and holographic microscopic imaging to these curcumin analogues for anti-proliferative activity against A549 and H1975 lung adenocarcinoma cells. The TNFα-induced NF-κB inhibition and autophagy induction effects correlated strongly with the cytotoxic potential of the analogues. Significant inhibition of tumour growth was observed when the most potent analogue 44 was added in liposomes at one-sixth of the maximally tolerated dose in the A549 xenograft model.

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  • History of Cell Culture
    M. Jedrzejczak-Silicka
    Biochemistry, Genetics and Molecular Biology » "New Insights into Cell Culture Technology" (2017)

    Book chapter review

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  • Hydroxylated Fluorescent Dyes for Live-cell Labeling: Synthesis, Spectra and Super-resolution Sted
    Chemistry — A European Journal (2017)

    Cell viability in the presence of dyes in the centromeres was verified by measuring cell morphology of Drosophila S2 cells using a holographic microscope (Holomonitor M4). images and measures cells without using invasive dyes or reagents. Using HoloMonitor, the authors proved that cells stay alive and continue to divide after staining them with the improved super-resolution dyes.

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  • Lipid Deregulation in UV Irradiated Skin Cells: Role of 25-hydroxy­cholesterol in Keratinocyte Differentiation During Photoaging
    The journal of steroid biochemistry and molecular biology (2017)

    Cell morphological changes in ageing: Skin photoaging due to UV irradiation is a degenerative process that appears more and more as a growing concern. Lipids, including oxysterols, are involved in degenerative processes; as skin cells contain various lipids, the aim of our study was to evaluate first, changes in keratinocyte lipid levels induced by UV exposure and second, cellular effects of oxysterols in cell morphology and several hallmarks of keratinocyte differentiation.

    Using HoloMonitor and confocal microscopy analysis, the authors highlighted cell thickening and cytoskeletal damage after incubation of keratino­cytes with 25-hydroxycholesterol.

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  • Applications of Label-free, Quantitative Phase Holographic Imaging Cytometry to the Development of Multi-specific Nanoscale Pharmaceutical Formulations
    Luther E et al
    Cytometry Part A 2017 (2017)

    A review of HoloMonitor applications: tracking of Giant HeLa cells, which may be undergoing neosis tracking the effects of cell cycle related toxic agents on cell lines; using MicroRNAs to reverse the polarization state in macrophages to induce tumor cell killing development of liposomal nanoformulations to overcome multi-drug resistance in ovarian cancer cells and development of dual sensitive micelles to specifically target matrix metalloproteinase 2

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  • Label-free High Temporal Resolution Assessment of Cell Proliferation Using Digital Holographic Microscopy
    Cytometry Part A 2017 (2017)

    The authors have developed a robust and label-free kinetic cell proliferation assay with high temporal resolution for adherent cells using HoloMonitor M4. Only two image processing settings were adjusted between cell lines, making the assay practical, user friendly, and free of user bias. In the recorded time-lapse image sequences, individual cells were automatically identified to provide detailed growth curves and growth rate data of cell number, confluence, and average cell volume. The results demonstrate how these parameters facilitate a deeper understanding of cell processes than what is achievable with current single-parameter and end-point methods.

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  • Characterization of a Transgenic Mouse Model Exhibiting Spontaneous Lung Adenocarcinomas With a Metastatic Phenotype
    Hsuen-Wen Chang, Zih-Miao Lin, Min-Ju Wu, Li-Yu Wang, Yen-Hung Chow, Shih Sheng Jiang, Hui-Ju Ch’ang and Vincent HS Chang
    PLOS ONE (2017)

    The authors have developed a mouse model which is very similar to human, and which can be used for more relevant drug evaluations. Focus are is tumor metatasis and especially the phenomenon of the epithelial-mesenchymal transition (EMT). HoloMonitor was used to investigate the capability of cell movement of tumor cells at different stages.

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  • The “inherent Vice” in the Anti-angiogenic Theory May Cause the Highly Metastatic Cancer to Spread More Aggressively
    Denian Wang, Chun Tan, Fei Xiao, Lan Zou, Lijun Wang, Yong’gang Wei, Hanshuo Yang, and Wei Zhang
    Scientific Reports (2017)

    Even if the blood supply is cut off in tumors, cells will survive, and even migrate, and maybe cause increased metastasis.

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  • Quantitative Phase-contrast Imaging – a Potential Tool for Future Cancer Diagnostics
    Anette Gjörloff-Wingren
    Cytometry Part A (2017)

    Prof. Gjörloff-Wingren has used holographic microscopy in cancer related research for over a decade. Gjörloff-Wingren discuss the emerging field of quantitative phase imaging and the novel label-free capabilities that promise to improve and succeed invasive laborious photochemical procedures

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  • In Situ Morphological Assessment of Apoptosis Induced by Phaleria Macrocarpa (Boerl.) Fruit Ethyl Acetate Fraction (Pmeaf) in Mda-mb-231 Cells by Microscopy Observation
    Nowroji Kavitha, Yeng Chen, Jagat R. Kanwar and Sreenivasan Sasidharan
    Biomedicine & Pharmacotherapy (2017)

    The purpose of the study was to determine the cytotoxic effect of P. macrocarpa fruit ethyl acetate fraction (PMEAF) by using various conventional and modern micro­scopy techniques. The results indicate that PMEAF is a potential anticancer agent and a candidate for developing more potent anticancer drugs.

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  • Promising Anticancer Activity of Cyclotrichium Niveum L. Extracts Through Induction of Both Apoptosis and Necrosis
    Aysun Özdemir, Metin Yildiz, Fatma Sezer Senol, Yaprak Dilber Şimay, Burçin Ibişoglu, Alper Gokbulut, Ilkay Erdogan Orhan and Mustafa Ark
    Food and Chemical Toxicology (2017)

    Aim was to study the impact of plant extracts from Cyclotrichium niveumon on normal-like and cancer cell lines. The authors concluded that C. niveum has cytotoxic properties in the cancer cell lines tested.

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  • Characterization of Dormant and Active Human Cancer Cells by Quantitative Phase Imaging
    Peng Guo, Jing Huang and Marsha A. Moses
    Cytometry Part A (2017)

    Based on HoloMonitor M4, the authors developed a novel in vitro method to characterize the angiogenic and non-angiogenic phenotypes of human osteosarcoma cells. Compared with traditional in vitro assays, the approach provides a non-invasive and low-cost method to assess a panel of cell morphology and cell behaviors. The authors were able to easily and accurately differentiate between the angiogenic and non-angiogenic phenotypes of KHOS cells by comparing five cellular parameters: cell area, cell thickness, cell migration, cell motility and cell motility speed.

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  • Synthesis, Characterization and in Vitro and in Vivo Photodynamic Activities of a Gallium(III) Tris(Ethoxycarbonyl)corrole
    Zhao Zhang, Hua-Hua Wang, Hua-Jun Yu, Yu-Zhen Xiong, Hai-Tao Zhang, Liang-Nian Jid and Hai-Yang Liu
    Dalton Transactions (2017)

    After illumination and treatment with 1-Ga, A549 cells exhibited characteristics typical of apoptosis, spherical shape of the cells, chromatin condensation, and nuclear membrane irregularity. After incubation for 1 h, the authors found that the cells started to contract, the area decreased and the thickness increased.

    Read more
  • Holography: the Usefulness of Digital Holographic Microscopy for Clinical Diagnostics
    Holographic Materials and Optical Systems 2017 (2017)

    Open access book chapter discussing the potential of holographic microscopy in clinical applications

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  • Loss of Erα Induces Amoeboid-like Migration of Breast Cancer Cells by Downregulating Vinculin
    Yuan Gao, Zhaowei Wang, Qiang Hao, Weina Li, Yujin Xu, Juliang Zhang, Wangqian Zhang, Shuning Wang, Shuo Liu, Meng Li, Xiaochang Xue, Wei Zhang, Cun Zhang and Yingqi Zhang
    Nature Communications (2017)

    The authors tracked and analyzed the migration of different MCF-7 cell populations in a 3D matrix. In addition to measuring the cell migration speed with HoloMonitor, the following five phenotypes were observed: ‘stable adhesion’, ‘unstable lamellipodia’, ‘bleb’, ‘stable bleb’ and ‘unstable pseudopod’.

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  • Three-dimensional Morphology of C6/36 Cells Infected by Dengue Virus: a Study Based on Digital Holographic Microscopy
    Yu JH, Liu XL, Liu YJ, He XE, Hui Y, Zhang B, Zhu L and Zhao W
    Journal of Southern Medical University (2017)

    Digital holographic microscopy allows monitoring of the complex morphological changes of cells during DENV infection. The 4 DENV strains with different serotypes causes characteristic cell damages during infection

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  • Supervised Classification of Etoposide-treated in Vitro Adherent Cells Based on Noninvasive Imaging Morphology
    Anna Leida Mölder, Johan Persson, Zahra El-Schich, Silvester Czanner, Anette Gjörloff-Wingren
    Journal of Medical Imaging (2017)

    The morphological changes observed occur before and at lower concentrations than a reduction in cell metabolic activity or viability. Three classifiers are compared and we report a best case sensitivity of 88% and specificity of 94% for classification of cells as treated/untreated.

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  • Cathelicidins Positively Regulate Pancreatic Β-cell Functions
    J. Sun, M. Xu, H. Ortsäter, E. Lundeberg, L. Juntti-Berggren, Y. Q. Chen, J. Z. Haeggström, G. H. Gudmundsson, J. Diana and B. Agerberth
    The faseb journal (2016)

    HoloMonitor M4 was used to assess the cell density during cell proliferation.

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  • Non-invasive, Quantitative Assessment of the Morphology of y-irradiated Human Mesenchymal Stem Cells and Periosteal Cells Using Digital Holographic Microscopy
    International journal of radiation biology (2016)

    Cell morphological parameters (e.g. cell volume) monitored by HoloMonitor could be useful and more stable than the DNA damage markers currently used.

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  • Reversal of Chemoresistance in Ovarian Cancer by Co-delivery of a p-glycoprotein Inhibitor and Paclitaxel in a Liposomal Platform
    Molecular cancer therapeutics (2016)

    To follow the development of mitotic arrest followed by apoptosis in real time.

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  • Ca 2+ Signals, Cell Membrane Disintegration, and Activation of tmem16f During Necroptosis
    Cellular and molecular life sciences (2016)

    It is well known that cells swell during necroptosis. However, proper cell volume measurements are still missing. Using Holo­Monitor M4 the authors non-invasively measured the cell volume changes associated with necroptosis. The results were confirmed by flow cytometry measurements.

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  • Relationship Between tmem16a/anoctamin 1 and lrrc8a
    European journal of physiology (2016)

    Analysis of hypo-induced changes in cell height rather than cell volume, as an increase in cell height is not compromised by basal adhesions or cell/cell contacts. Cell height measured by holographic microscopy in HEK293 cells, indicated hypotonic cell swelling (Hypo) within seconds and completed regulatory volume decrease (RVD) within less than 2 min

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  • Mixed Nanosized Polymeric Micelles as Promoter of Doxorubicin and Mirna-34a Co-delivery Triggered by Dual Stimuli in Tumor Tissue
    Guseppina Salzano, Daniel F. Costa, Can Sarisozen, Ed Luther, George Mattheolabakis, Pooja P. Dhargalkar, and Vladimir Torchilin
    Small (2016)

    Methods for producing 4D plot were developed. Images from the time course were exported to ImageJ and treated as an image stack, creating a volume rendering of the cellular motion over time of analysis. The brightness threshold was adjusted to view all cells within the imaging area or only the thicker cells, a feature that allows identification of mitosis, apoptosis, and transition between mesenchymal and amoeboid cellular morphologies

    Read more
  • Biophysical Characteristics of Proteins and Living Cells Exposed to the Green Tea Polyphenol Epigallocatechin-3-gallate
    Beatrix Peter, Szilvia Bosze and Robert Horvath
    European biophysics journal (2016)

    HoloMonitor-based methods and other methods to assess cell adhesion, motility, migration, stiffness, apoptosis and proliferation exposed to green tea polyphenol epigallocatechin-3-gallate are reviewed.

    Read more
  • Label-free Optical Biosensors for Monitoring Cellular Processes and Cytotoxic Agents at Interfaces Using Guided Modes and Advanced Phase-contrast Imaging Techniques
    Inna Székács, Robert Horvath and András Székács
    Advanced sciences and technologies for security applications (2016)
  • Ion Channels in Regulated Cell Death
    Cellular and molecular life sciences (2016)

    Despite the rather different morphologic appearances of apoptosis and necroptosis, both mechanisms of cell death require the transport of ions, organic osmolytes, and water. The contribution of these transport proteins and underlying mechanisms are summarized in this review.

    Read more
  • Shielding of the Geomagnetic Field Alters Actin Assembly and Inhibits Cell Motility in Human Neuroblastoma Cells
    W-C. Mo, Z-J. Zhang, D-L. Wang, Y. Liu, P. Bartlett and R-Q. He
    Scientific reports (2016)

    Using holomonitor M4, the authors show that the elimination of Earth’s magnetic field affects cell behavior. Potential influence on cancer treatments suggested.

    Read more
  • Pseudomonas Aeruginosa n-3-oxo-dodecanoyl-homoserine Lactone Elicits Changes in Cell Volume, Morphology, and aqp9 Characteristics in Macrophages
    A. Holm, K-E. Magnusson and E. Vikström
    Frontiers in cellular and infection microbiology (2016)

    Analysis of optical cell volume changes; time-lapse videos were recorded using the holomonitor M4 Image acquisition was set at an interval of 30 s for 1 h.

    Read more
  • The Curcumin Analog c-150, Influencing nf-κb, upr and akt/notch Pathways Has Potent Anticancer Activity in Vitro and in Vivo
    Plos one (2016)

    Visualization of cytotoxic effects using holomonitor. These experiments record morphological parameters of treated cells such as area, thickness and volume. First time where the effects of drug induced cell death on cellular morphology of these glioma cells were detected by using 3D holographic images.

    Read more
  • Incubator Proof Miniaturized Holomonitor to in Situ Monitor Cancer Cells Exposed to Green Tea Polyphenol and Preosteoblast Cells Adhering on Nanostructured Titanate Surfaces: Validity of the Measured Parameters and Their Corrections
    Beatrix Peter, Judit Nador, Krisztina Juhasz, Agnes Dobos, Laszlo Korosi, Inna Székács, Daniel Patko and Robert Horvath
    J. Biomed. Opt (2015)

    Holomonitor M4 was used to observe cancer cell motility, motility speed, and migration in the presence of the green tea polyphenol, epigallocatechin gallate, as well as to monitor the adhesion of preosteoblast cells on nanostructured titanate coatings, relevant for biomedical applications.

    Read more
  • Enhanced Cytotoxicity of Folic Acid-targeted Liposomes Co-loaded With C6 Ceramide and Doxorubicin: in Vitro Evaluation on HeLa, A2780-ADR and H69-AR Cells
    S. K. Sriraman, J. Pan, C. Sarisozen, E. Luther and V. Torchilin
    Molecular pharmaceutics (2015)

    Using holomonitor M4, it was found that FA-(C6+Dox)-LP treated hela cells underwent rapid progression to apoptosis (cell suicide) after 21 hours, as evidenced by a drastic drop in cell area after loss of cell membrane integrity.

    Read more
  • From Immobilized Cells to Motile Cells on a Bed-of-nails: Effects of Vertical Nanowire Array Density on Cell Behavior
    H. Persson, Z. Li, J. O. Tegenfeldt, S. Oredsson and C. N. Prinz
    Scientific reports (2015)

    Under­standing of the inter­actions between cells and nanowires. The results provide guidelines to minimize cellular stress on nanowire arrays. Additionally, the findings show that cell behavior can be controlled by adjusting nanowire density, which may have applications in drug development.

    Read more
  • Targeted Tumor Therapy by Rubia Tinctorum L.: Analytical Character­ization of Hydroxyan­thraquinones and Investigation of Their Selective Cytotoxic, Adhesion and Migration Modulator Effects on Melanoma Cell Lines
    E. Lajkó, P. Bányai, Z. Zámbó, L. Kursinszki, É. Szőke and L. Kőhidai
    Cancer cell international (2015)

    Our results highlight the effective and selective inhibitory activity of purpurin towards melanoma cells and its possible use as a targeted anticancer agent. The anthraquinones of the cytotoxic extract are suggested to apply in drug delivery systems as an anticancer drug

    Read more
  • Poloxamer Bioadhesive Hydrogel for Buccal Drug Delivery: Cytotoxicity and Trans-epithelial Permeability Evaluations Using Tr146 Human Buccal Epithelial Cell Line
    N. Zeng, N. Mignet, G. Dumortier, E. Olivier, J. Seguin, M. Maury, D. Scherman, P. Rat and V. Boudy
    International journal of pharmaceutics (2015)

    The assessment of cell viability (MTT, Alamar Blue), membrane integrity (Neutral Red), and apoptosis assay (Hoechst 33342), were performed and associated to holomonitor M3 analysis.

    Read more
  • See in 3D: state of the art of 3D display technologies
    Yang L, Dong H, Alelaiwi A, and El Saddik A
    Multimed Tools Appl (2015)

    Review introducing the principles of current 3D display technologies and their associated applications. HoloMonitor is shown and discussed as an example of Digital Holographic Microscopy (DHM). Advantages with DHM are described and the reasons why the technique is label-free explained.

    Read more
  • Reversal of Chemoresistance in Ovarian Cancer by Co-delivery of MDR Inhibitors and Paclitaxel Using a Targeted Liposomal Platform
    Shravan Kumar Sriraman, Yilin Zhang, Ernst Lengyel and Vladimir Torchilin
    The faseb journal (2015)

    Using time-course holographic imaging studies, this cell cycle arrest followed by progression to apoptosis was clearly visualized and confirmed.

    Read more
  • Macrophage Polarization and the Effect of Microrna-155 Administered in Water-in-oil-in-water Multiple Emulsion Formulations
    Oka A, Talekar M, Ouyang Q, Luther E and Amiji M
    Journal of Clinical & Cellular Immunology (2015)

    Holographic assessment using Holomonitor M4 of the co-cultured cells in real-time showed differences in motility and morphology of macrophages with mir-155 ME treated cells showing greater cellular interaction between the two phenotypes.

    Read more
  • Interfacing Antibody-based Microarrays and Digital Holography Enables Label-free Detection for Loss of Cell Volume
    Zahra El-Schich, Emmy Nilsson, Anna S Gerdtsson, Christer Wingren and Anette Gjörloff Wingren
    Future science oa (2015)

    The article provides proof of concept for using holographic microscopy combined with antibody-based microarray technology for detecting morphological changes in captured cells.

    Read more
  • Induction of Morphological Changes in Death-induced Cancer Cells Monitored by Holographic Microscopy
    Zahra El-Schich, Anna Mölder, Helena Tassidis, Pirkko Härkönen, Maria Falck Miniotis and Anette Gjörloff Wingren
    Journal of structural biology (2015)

    Using HoloMonitor M3, the authors show that holographic microscopy allows label-free and completely non-invasive morphological measurements of cell growth, viability and death.

    Read more
  • Environmental and Toxicological Impacts of Glyphosate With Its Formulating Adjuvant
    I. Székács et al.
    International Journal of Agricultural, Biosystems Science and Engineering (2014)

    The article describes a study of the health effects of the well-known, widely used herbicide Roundup. In the study, the HoloMonitor M4 was used to assess toxic effects to animal cells after exposure to Roundup.

    Read more
  • Digital Holographic Microscopy for Non-invasive Monitoring of Cell Cycle Arrest in L929 Cells
    Maria Falck Miniotis, Anthonny Mukwaya and Anette Gjörloff Wingren
    PLOS ONE (2014)

    We show that average cell phase volume results from DHM readings are comparable to the flow cytometry findings. DHM thus provides a non-disruptive alternative to flow cytometry. The technique has the potential to develop into a fast and cost-efficient method for pre-clinical monitoring of cancer treatment efficacy.

    Read more
  • Combination of Unsaturated Fatty Acids and Ionizing Radiation on Human Glioma Cells: Cellular, Biochemical and Gene Expression Analysis
    Otilia Antal, László Hackler, Junhui Shen, Imola Mán, Katalin Hideghéty, Klára Kitajka and László G Puskás
    Lipids in health and disease (2014)

    Holomonitor M3 was used to assess the increased death of glioma (brain tumor) cells when combining irradiation with various unsaturated fatty acid treatments

    Read more
  • The Role of Pi3k/akt-related Pip5k1α and the Discovery of Its Selective Inhibitor for Treatment of Advanced Prostate Cancer
    J. Semenas et al
    PNAS (2014)

    The Holomonitor M3 imaging system was applied to capture living PC-3 cells in culture during treatment

    Read more
  • Edaravone Protects Against Methylglyoxal-induced Barrier Damage in Human Brain Endothelial Cells
    Andrea E. Tóth et al
    PLOS ONE (2014)

    Using holographic microscopy for label-free analysis of living cells, visualized for the first time the kinetics of morphological changes caused by methylglyoxal. The morphology data confirmed that edavarone completely prevented methylglyoxal-induced changes in morphology and detachment of cells.

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  • Protein kinase A (PKA) pathway is functionally linked to androgen receptor (AR) in the progression of prostate cancer
    M Sarwara, S Sandberg, P-A Abrahamsson, and J L Persson
    Urologic Oncology (2014)

    Aim of the study was to explore a suggested signalling pathway in the progression of prostate cancer. HoloMonitor M3 was used for imaging of cells and for analysis of morphological parameters.

    Read more
  • Fibroblasts Cultured on Nanowires Exhibit Low Motility, Impaired Cell Division, and Dna Damage
    H. Persson, C. Købler, K. Mølhave, L. Samuelson, J. O. Tegenfeldt, S. Oredsson and C. N. Prinz
    Small (2013)

    Fibroblast behaviour on vertical nanowire arrays was investigated using the Holo­Monitor M3. It was shown that cell motility and proliferation rate are reduced on nanowires. Fibroblasts cultured on long nanowires exhibit failed cell division, DNA damage, increased ROS content and respiration.

    Read more
  • Cells and Holograms — Holograms and Digital Holographic Microscopy as a Tool to Study the Morphology of Living Cells
    K. Alm, Z. El-Schich, M. Falck Miniotis, A. Gjörloff Wingren, B. Janicke and S. Oredsson
    Holography — Basic Principles and Contemporary Applications  (2013)
  • Lipids as Tumoricidal Components of Human Α-lactalbumin Made Lethal to Tumor Cells (Hamlet): Unique and Shared Effects on Signaling and Death
    J. C. S. Ho, P. Storm, A. Rydström, B. Bowen, F. Alsin, L. Sullivan, I. Ambite, K. H. Mok, T. Northen and C. Svanborg
    J. Of Biological Chemistry (2013)

    The authors used the holomonitor M3 to observe morphological differences on cells over time, treated with either Hamlet or various lipids associated with HAMLET.

    Read more
  • A Unifying Mechanism for Cancer Cell Death Through Ion Channel Activation by Hamlet
    P. Storm, T. K. Klausen, M. Trulsson, J. C. S Ho, M. Dosnon, T. Westergren, Y. Chao, A. Rydström, H. Yang, S. F. Pedersen and C. Svanborg
    PLOS One (2013)

    Changes in cell morphology was studied using HoloMonitor technology

    Read more
  • Prevention and Treatment of Colon Cancer by Peroral Administration of Hamlet
    M. Puthia, P. Storm, A. Nadeem, S. Hsiung, C. Svanborg
    Gut (2013)

    Using the HoloMonitor M2, the authors show that cell thickness and adherent surface area change drama­tically after HAMLET treatment. HAMLET is a human α-lactalbumin made lethal to tumor cells.

    Read more
  • Low Resolution Solution Structure of Hamlet and the Importance of Its Alpha-domains in Tumoricidal Activity
    J. C. S. Ho, A. Rydström, M. S. S. Manimekalai, C. Svanborg and G. Grüber
    PLOS ONE (2012)

    Peptides covering the alpha1 and alpha2 domains of the protein triggered rapid ion fluxes in the presence of sodium oleate and were internalized by tumor cells, causing rapid and sustained changes in cell morphology. The alpha peptide-oleate bound forms also triggered tumor cell death with comparable efficiency as HAMLET. In addition, shorter peptides corresponding to those domains are biologically active.

    Read more
  • Hamlet: Functional Properties and Therapeutic Potential
    J. C. S. Ho, A. Rydström, M. Trulsson, J. Bålfors, P. Storm, M. Puthia, A. Nadeem and C. Svanborg
    Future Medicine (2012)
  • Dermatan Sulfate Is Involved in the Tumorigenic Properties of Esophagus Squamos Cell Carcinom
    M. A. Thelin, K. J. Svensson, X. Shi et al
    Cancer Research (2012)

    Chondroitin and dermatan sulfate proteoglycans, which occur both in the matrix and at the cell surface, play important roles in thetumorgenic processes. The unique feature that distinguishes DS from CS is the presence of iduronic acid (IdoA) in DS. IdoA in DS influences tumorigenesis by affecting cancer cell behavior

    Read more
  • Hamlet Binding to Α-actinin Facilitates Tumor Cell Detachment
    M. Trulsson, H. Yu, L. Gisselsson, Y. Chao, A. Urbano, S. Aits, A-K. Mossberg, C. Svanborg
    PLOS ONE (2011)

    Interaction between HAMLET and a-actinins promotes tumor cell detachment.

    Read more
  • Digital Holography and Cell Studies
    K. Alm, H. Cirenajwis, L. Gisselsson, A. Gjörloff Wingren, B. Janicke, A. Mölder, S. Oredsson, J. Persson
    Holography-Research and technologies (2011)
  • Generation of Trisomies in Cancer Cells by Multipolar Mitosis and Incomplete Cytokinesis
    David Gisselsson Nord et al.
    PNAS (2010)

    Gisselsson and colleagues studied cell division in short-term cultures established from fibroblasts and primary tumors. The researchers showed that abnormal cell divisions can cause an abnormal number of chromosomes in cancer cells.

    Read more
  • Reduction of the Putative CD44+CD24 Breast Cancer Stem Cell Population by Targeting the Polyamine Metabolic Pathway With Pg11047
    Cirenajwis et al.
    Anticancer Drugs (2010)

    Cirenajwis and colleagues used the label-free live cell imaging capabilities of the HoloMonitor to study the movement of JIMT-1 breast cancer cells. They showed that treatment with the ornithine analogue difluoromethylor-nithine (DFMO) increased cell motility, while treatment with the polyamine analogue PG-11047 decreased cell motility.

    Read more
  • Digital Holography and Cell Motility
    J. Persson, A. Mölder, S-G. Pettersson, K. Alm
    Microscopy: Science, Technology, Applications and Education (2010)

    Using digital holography in combination with our program Celltrack, we can follow a population of cells, and we can study single cells within that population. The results also show that we can correlate cell movement with the level of confluence in the cultures, cell area, cell optical thickness and cell volume. In all, digital holography proved suitable for a cell movement assay.

    Read more
  • Immunomodulatory Nanoparticles as Adjuvants and Allergen-delivery System to Human Dendritic Cells: Implications for Specific Immunotherapy
    S. Broos, K. Lundberg, T. Akagi, K. Kadowaki, M. Akashi, L. Greiff, C. A. Borrebaeck, M. Lindstedt
    Vaccine (2010)
  • Digital Holographic Microscopy — Innovative and Non-destructive Analysis of Living Cells
    Z. El-Schich, A. Mölder, M. Sebesta, L. Gisselsson, K. Alm, A. Gjörloff Wingren
    Microscopy: Science, Technology, Applications and Education (2010)
  • Non-invasive, Label-free Cell Counting and Quantitative Analysis of Adherent Cells Using Digital Holography
    Anna Mölder et al
    Journal of Microscopy (2008)

    Using a unique non-invasive labelfree cell counting method, results comparable to conventional cell counting using a haemocytometer were produced. The major advantage using HolomonitorTM M2 is the opportunity to easily access information about cell number, size, optical thickness and confluence in an automatic, non-invasive manner.

    Read more
  • A Hardware Acceleration Platform for Digital Holographic Imaging
    T. Lenart, M. Gustafsson, V. Öwall
    Journal of VLSI Signal Processing Systems (2008)
  • Modeling and Exploration of a Reconfigurable Architecture for Digital Holographic Imaging
    Thomas Lenart, Henrik Svensson and Viktor Öwall
    IEEE (2008)
  • Object Characterization With Refractometric Digital Fourier Holography
    M. Sebesta, M. Gustafsson
    Optics Letters (2005)
  • High-resolution Digital Transmission Microscopy — a Fourier Holography Approach
    M. Gustafsson, M. Sebesta, B. Bengtsson, S.G. Pettersson, P. Egelberg, T. Lenart
    Optics and Lasers in Engineering (2004)
  • Refractometry of Microscopic Objects With Digital Holography
    M. Gustafsson, M. Sebesta
    Applied Optics (2004)

Conference Papers and Posters

  • Comparison of the effects of pharmaceutical compounds on tumor cells in 2D and 3D in vitro models using label-free, quantitative 4 dimensional holo­graphic imaging
    Ed Luther, Giuseppina Salzano, Shravan Kumar Sriraman, Daniel Costa and Vladimir P. Torchilin
    AACR-NCI-EORTC Inter. Conf. on Molecular Targets and Cancer Therapeutics 2015 (2018)
  • Development of a 4-Sample Version of the Kolmogorov-Smirnov Test for Evaluating the Temporal Physiology of Cells Treated with test Compounds in a Label-Free, High Content, Platform for Quantitative Analysis of Adherent Cell-Culture Models
    Ed Luther, Livia Mendes, Jiayi Pan, Daniel Costa, Elena Holden and Vladimir Torchilin
    AACR Annual Meeting (2017)
    4-dimensional plots of HoloMonitor time-lapse data were developed to assist in the visualization of the effects of pharmaceutical compounds under development.
  • Cystatin C Decreases Proliferation of Melanoma Cells by Affecting Mitosis Length
    Hanna Wallin, Kersti Alm and Magnus Abrahamson
    AACR Annual Meeting (2017)
    Analysis using HoloMonitor revealed qualitative differences in the normal behavior of the A375 cells under study as a result of cystatin C addition. The motility of the cells was significantly affected. The mitosis phase was considerably prolonged in cells grown in cystatin C containing medium, indicating that the proliferation-inhibiting effect of the protease inhibitor may be through its direct action on proteolytic processes in the mitosis phase.
  • Transcription Regulation of Chemokine Receptor CXCR4 by Nuclear Respiratory Factor 1 (NRF1) Controls Estrogen-Induced Malignant Transformation of Breast Epithelial Cells to Breast Cancer Stem Cells
    J. K. Das and D. Roy
    AACR Annual Meeting (2016)
    Chemokine receptor CXCR4 is involved in the maintenance of stemness property of stem cells, aging and metastasis of cancer. NRF1 is one of the major transcription factors that controls transcription of CXCR4. In this study, the authors have investigated whether transcription regulation of CXCR4 by NRF1 controls estrogen-induced malignant transformation of breast epithelial cells to breast cancer stem cells.
  • A Label-Free, High Content, Moderate Throughput Analytical Platform for Quantitative Kinetic Analysis of Cell Behavior upon Drug Activation Based on the Kolmogorov-Smirnov Test
    Ed Luther, Livia Mendes, Jiayi Pan, Daniel Costa, Elena Holden and Vladimir Torchilin
    3rd Conference on Impedance-Based Cellular Assays (2016)
    “Our objective is to develop multi-functional nanotechnology-based antitumor drug delivery systems for improving efficacy of treatments and reducing undesirable side effects. The essential part of this process is the development of un-biased quantitative analytical techniques. We are reporting a successful validation of a very high content, medium throughput system in multi-well plates, employing the newly developed holographic imaging cytometer HoloMonitor® M4 for label-free time-lapse cellular analysis.”

    View poster

  • Impedance-Based Analysis and Holographic Phase Imaging of The GNRH-Iii-Based Drug-Targeting In Melanoma Cells
    E. Lajkó, S. Spring, O. Láng, S. Ingebrandt, G. Mező and L. Kőhidai
    3rd Conference on Impedance-Based Cellular Assays (2016)

    HoloMonitor M4 was used for the morphometry and migration studies.

    View paper

  • Characterization of macrophage behavior by 4-dimensional label free quantitative holographic imaging
    Ed Luther, Meghna Talekar, Adwait Oka, Qijun Ouyang, Mansoor Amiji.
    AACR Annual Meeting (2015)
  • Four dimensional quantitative label-free holographic imaging of the cell cycle in tumor cell lines
    Ed Luther, Jeffrey Agar and Mansoor Amiji
    AACR Annual Meeting (2015)

Doctoral Theses

  • Characterization of the Novel Pericyte Receptors S1PR3 and PTGER2
    Faculty of Biosciences, Heidelberg University
    Tina Heumann (2017)
    Morphological changes were evaluated by comparing time-plots of cell volume and thickness, created by HoloMonitor M4.
    Read more
  • Proteins, Polymers and Living Cells on Titanium Oxide-based Nano structured Coatings: Exploitation of Label-free Optical Sensing Methods
    Institute of Technical Physics and Material Science, Center for Energy Research, Hungarian Academy of Sciences
    Judit Nádor (2016)

    The HoloMonitor M4 was used to monitor living cells (human embryonic kidney cells and preosteoblasts) on nanostructured coatings in a novel cuvette configuration.

    Read more
  • Aquaporins in Infection and Inflammation
    Clinical and Experimental Medicine, Linköping University
    Angelika Holm (2016)
    By recording time-lapse videos directly within the incubator using HoloMonitor M4, volume changes of bacteria infected cells was studied.
    Read more
  • Novel Imaging Technology and Tools for Biomarker Detection in Cancer
    Health and Society, Malmö University
    Zahra El-Schich (2016)
    To analyze morphological changes of death cells, HoloMonitor was used. Digital holographic microscopy is an approach for label-free non-invasive 3D imaging of cultured cells. We have analyzed cell death of adherent cancer cells using HoloMonitor and developed it to analyze suspension cells by combining this technique with antibody based micro­assays. HoloMonitor can be used for cell-death induced cell analysis of both adherent cells and suspension cells.
    Read more
  • Combination of Unsaturated Fatty Acids and Ionizing Radiation on Human Glioma Cells: Cellular, Biochemical and Gene Expression Analysis
    Department of Pharmaceutical Technology, University of Szeged
    Otilia Antal (2016)
    HoloMonitor M3 was used to assess the increased death of glioma (brain tumor) cells when combining irradiation with various unsaturated fatty acid treatments.
    Read more
  • Modulation of Tight Junctions by Peptides to Increase Drug Penetration Across Biological Barriers
    Department of Pharmaceutical Technology, University of Szeged
    Alexandra Bocsik (2016)
    Morphological changes in peptide treated Caco-2 epithelial cells were analyzed using HoloMonitor technology.
    Read more
  • Protection of the Blood-Brain Barrier under Pathological Conditions
    University of Szeged, Faculty of Medicine and Biological Research Centre Hungarian Academy of Sciences, Szeged
    Andrea Tóth (2014)

    Endothelial cells were cultured on collagen coated culture dishes with borosilicate glass bottom. HoloMonitor was used to analyze cell morphological changes before and during treatments.

    Read more
  • Nanowires in Cell Biology – Exploring Interactions and Applications
    Division of Solid State Physics, Lund University
    Henrik Persson (2014)
    Using HoloMonitor M4 this thesis explores the interactions between cells and nanowires, to increase our under­standing of how cells are affected and how they can be manipulated by these one-dimensional, semiconductor, crystals.
    Read more
  • Design of Reconfigurable Hardware Architectures for Real-Time Applications
    Department of Electrical and Information Technology, Lund University
    T. Lenart (2008)