Board of Directors
Phase Holographic Imaging AB (PHI) provides researchers in academia and the life sciences industry with products for live-cell kinetic studies. PHI’s time-lapse cytometer, the HoloMonitor® system, is based on our core technology holographic microscopy. Since introducing the first instrument in 2011, PHI now offers a range of products for the label-free, long-term quantitative analysis of living cell dynamics, circumventing the drawbacks of traditional methods requiring toxic stains. Headquartered in Lund, Sweden, PHI trades through a network of international distributors.
The HoloMonitor system is a versatile tool providing detailed information about the life cycle and behavior of living, or dying, cells to scientists all over the world. Focus areas include basic cell research as well as the development of more efficient treatment strategies for cancer and other diseases. Committed to promoting the science and practice of time-lapse cytometry, PHI is continuously developing new products and applications in close collaboration with scientific expertise.
Modern drug research is based on experiments performed on cultured cells. Living cells are translucent and therefore difficult to see in an ordinary light microscope without stains or labels. Many of the methods used to visualize cells are destructive. This has curiously meant that life’s smallest building block – the cell – is generally studied only when it is dead.
The concept of holographic microscopy
PHI’s time-lapse cytometers are designed to non-invasively explore the kinetics of cellular responses, rather than just providing snapshots the way traditional endpoint assays do. PHI’s time-lapse cytometers are based on a novel microscopy technology – holographic microscopy – that automatically analyzes living cells in ways not previously possible.
PHI has been granted over 10 patents and has a portfolio of further applications in the pipeline. This new digital capability will fundamentally change light microscopy and thus humanity’s ability to observe living cells.
History – Development of a successful product line
After successful collaboration between the Competence Center for Circuit Design (CCCD) at Lund University and PHI’s founders resulting in M1, the first prototype of the HoloMonitor product line, PHI was founded in 2004.
PHI’s first commercial product, the HoloMonitor M3, was launched on the international market in 2011. The HoloMonitor M3 (red and grey instrument in the photo above) is a bench-top time-lapse cytometer equipped with a motorized stage and variable magnification. In the same year, PHI received a governmental grant from Vinnova to develop the fourth HoloMonitor generation.
As a result, PHI’s second product, the HoloMonitor M4 (blue and white instrument in the photo above), was internationally launched in early 2013. The HoloMonitor M4 allows for the time-lapse analysis of living cells directly in the cell incubator in which they are being cultured.
Today, HoloMonitor systems are in use in Europe, North America, Asia, and Australia and their uses and benefits have been described in a long series of peer-reviewed scientific publications.
Board of Directors
Former CEO of Biosciences Group Thermo Fisher Scientific.
CEO of In Vitro Diagnostics and Research & Applied Markets GE Healthcare. Former CEO of GenVec and Group V.P. of Cellular Analysis Beckman-Coulter.
Former CEO of the pioneer of laser scanning cytometry – CompuCyte, Boston USA.
PHI collaborates with a range of centers of excellence, each running first-class research programs in which HoloMonitor technology plays an important role. Joint programs include activities intended to establish the best practices and validate the most useful HoloMonitor parameters:
- Develop novel applications based on HoloMonitor technology
- Establish a network of collaborators utilizing holographic imaging cytometry
- Improve methods for the clinical diagnosis of cancer
Results from our partner centers of excellence are presented at scientific meetings and published in peer-reviewed scientific journals. We also organize technology symposia and hands-on laboratory courses.