- Last Updated on Monday, 04 May 2015 10:10
Quantitative phase microscopy quantify variations in optical density, enabling living cell to be observed and quantified in ways not previously possible.
An ordinary light microscope can only detect variations in light intensity. Translucent objects, like unstained living cells, therefore have very poor contrast when observed with an ordinary microscope.
Living cells are more optically dense than the fluid cell culture media they are kept in. This makes it possible to observe living cells with good contrast by using a special kind of light microscope that visualizes variations in optical density – the phase contrast microscope.
However, to achieve contrast the phase contrast microscope creates visual artifacts. These artifacts make it difficult to computer process phase contrast images to extract quantitative information. Instead of creating artifacts, a quantitative phase microscope visualizes variations in optical density by displaying the optical thickness of an object in each image point.
Phase shift images
As living cells are more optically dense than their surroundings, they reduce the speed of the light wave that passes through them. The speed difference creates a dent in the uniform wave front that illuminates the cells.
A quantitative phase microscope quantifies the depth of the dent by measuring how much the phase of the light wave has been shifted when passing through the cells. The measured phase shift is displayed in a so called phase shift or phase image, where color or intensity variations represent the phase shift (top banner).
Optical thickness and volume
The measured phase shift is proportional to the optical thickness. By integrating the optical thickness over the cell area, the optical volume of a cell can be calculated. The ability to measure optical cell volume is unique to quantitative phase microscopy and cannot be done with conventional microscopy.
When a cell is dying, surrounding cell culture media enters the cell through the punctured cell membrane. This causes a gradual reduction in the cells optical density and optical volume. The health status of a cell culture can thus be assessed without stains by identifying cells with a gradual decrease in optical volume.