HoloMonitor M4 spacer plates

Cell culture vessels comes in many shapes and sizes. Unfortunately cell culture vessels are not standardized. The bottom and the bottom rim thickness varies between different brands and types. The unique software autofocusing will adjust the fine focus. However, different spacer plates must be used to adjust the coarse focus distance.

HoloMonitor M4 comes with four spacer plates of varying thickness:

Plate #Thickness
11.0 mm
21.5 mm
32.0 mm
42.5 mm

Later versions of the spacer plates are marked with the plate number. If the plate number is not visible, please measure the thickness and use the above table to identify the plate. The table below helps you to select the appropriate spacer plate for recommended cell culture vessels.

Cat. # Vessel Recommended Plate #
Nexcelom
CHT4-PD100-002 Cell counting chamber 3
BD Falcon
351 005 Petri 90 mm diameter 3
351 005 Petri 90 mm diameter 3
353 136 T75 culture flask No, rests on objective
Nunc
140685 6 well plate, Nunclon No, rests on objective
141008 24 well plate, Nunclon No, rests on objective
150350 92 mm TC dish (Petri), Nunclon Delta 2
153066 35 mm TC dish (Petri), Nunclon Delta 3
156367 T25 Easy flask, Nunclon Delta 1
156499 T75 Easy flask, Nunclon Delta No, rests on objective
176740 Multidish 4 well Nunclon Delta 1
Corning Costar
3516 6 well plate 1
430639 T25 culture flask 1
TPP
93100 Petri 90 mm diameter 1
93040 Petri 40 mm diameter 2
90075 T75 culture flask No, bottom slighly tilted
90025 T25 culture flask 2
SPL
T25 culture flask No, stripes in plastic bottom
BioLite
130181 Petri 60 mm culture dish 3
130184 6 well multidish No, rests on objective
130190 T75 Flask vented 1
130192 T25 Plug Seal 1
IBIDI
80406 µ-slide 4 well 3
80106 µ-slide 1 channel 4
81156 35 mm petri 3
Greiner bio-one
627898 Cellview glass bottom dish,
4 compartments
No
Sarstedt
83.3920 6-well plate none
83.3910 T25 2
94.6150.101 Chamber slide Lumox bottom 3
Shanghai Sunub Bio-tech
- 6-well plate No, socket too high
- Petri 35 mm 3
- T25 No, stripes on bottom