The publications and conference poster below have been published by customers or discuss applications of PHI's core technology – holographic and quantitative phase imaging (QPI) microscopy.

Peer reviewed articles and book chapters


A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells
Journal of Visualized Experiments 2018
Jing Huang, Peng Guo and Marsha A. Moses
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Ouabain suppresses the growth and migration abilities of glioma U‑87MG cells through inhibiting the Akt/mTOR signaling pathway and downregulating the expression of HIF‑1α
Molecular Medicine Report 2018
Xiao‑Sa Yang et al.
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β-Nitrostyrene derivatives attenuate LPS-mediated acute lung injury via the inhibition of neutrophil-platelet interactions and NET release
American Journal of Physiology-Lung Cellular and Molecular Physiology 2018
Yao-Wen Chang et al.
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Kynurenic acid and its derivatives are able to modulate the adhesion and locomotion of brain endothelial cells
Journal of Neural Transmission 2018
Lajkó E., Tuka B., Fülöp F. et al.
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History of Cell Culture
New Insights into Cell Culture Technology 2017
Magdalena Jedrzejczak-Silicka
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Tau Phosphorylation and Amyloid-β Deposition in the Presence of Formaldehyde
Formaldehyde and Cognition 2017
Jing Lu and Rongqiao He
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iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Management
Neurotherapeutics 2017
Shi-Xiang Cheng, Zhong-Wei Xu, Tai-Long Yi, Hong-Tao Sun, Cheng Yang, Ze-Qi Yu, Xiao-Sa Yang, Xiao-Han Jin, Yue Tu and Sai Zhang
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NatD promotes lung cancer progression by preventing histone H4 serine phosphorylation to activate Slug expression
Nature Communications 2017
Junyi Ju, Aiping Chen […] Quan Zhao
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Zinc enhances the cellular energy supply to improve cell motility and restore impaired energetic metabolism in a toxic environment induced by OTA
Scientific Reports 2017
Xuan Yang, Haomiao Wang, Chuchu Huang, Xiaoyun He, Wentao Xu, Yunbo Luo and Kunlun Huang
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The role of Vimentin in Regulating Cell Invasive Migration in Dense Cultures of Breast Carcinoma Cells
Nano Letters 2017
Yonatan Messica, Adi Laser-Azogui, Tova Volberg, Yair Elisha, Kseniia Lysakovskaia, Roland Eils, Evgeny Gladilin, Benjamin Geiger and Roy Beck
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Interleukin-6 blockade attenuates lung cancer tissue construction integrated by cancer stem cells
Scientific Reports 2017
Hiroyuki Ogawa, Michiyo Koyanagi-Aoi, Kyoko Otani, Yoh Zen, Yoshimasa Maniwa and Takashi Aoi
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Influence of Salinomycin Treatment on Division and Movement of Individual Cancer Cells Cultured in Normoxia or Hypoxia Evaluated with Time-Lapse Digital Holographic Microscopy
Cell Cycle 2017
Sofia Kamlund, Daniel Strand, Birgit Janicke, Kersti Alm and Stina Oredsson
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Combined activation of MAP kinase pathway and β-catenin signaling cause deep penetrating nevi
Nature Communications 2017
Iwei Yeh et al.
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High accuracy label-free classification of single-cell kinetic states from holographic cytometry of human melanoma cells
Scientific Reports 2017
Miroslav Hejna, Aparna Jorapur, Jun S. Song and Robert L. Judson
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CFTR supports cell death through ROS-dependent activation of TMEM16F (anoctamin 6)
European Journal of Physiology 2017
Filipa Simões, Jiraporn Ousingsawat, Podchanart Wanitchakool, Ana Fonseca, Inês Cabrita, Roberta Benedetto, Rainer Schreiber and Karl Kunzelmann
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Accumulation of Simulated Pathological Level of Formaldehyde Decreases Cell Viability and Adhesive Morphology in Neuronal Cells
Progress in Biochemistry and Biophysics 2017
Wang Jing, Zhou Jun, MO Wei-Chuan, He Ying-Ge, Wei Yan, He Rong-Qiao and Yi Fa-Ping
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Hypoxia-inducible factor-1 alpha is involved in RIP-induced necroptosis caused by in vitro and in vivo ischemic brain injury
Scientific Reports 2017
Xiao-Sa Yang, Tai-Long Yi, Sai Zhang, Zhong-Wei Xu, Ze-Qi Yu, Hong-Tao Sun, Cheng Yang, Yue Tu and Shi-Xiang
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Synthesis, characterization and in vitro and in vivo photodynamic activities of a gallium(III) tris(ethoxycarbonyl)corrole
Dalton Transactions 2017
Zhao Zhang, Hua-Hua Wang, Hua-Jun Yu, Yu-Zhen Xiong, Hai-Tao Zhang, Liang-Nian Jid and Hai-Yang Liu
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Biophysical characterization of nanostructured TiO2 as a good substrate for hBM-MSC adhesion, growth and differentiation
Experimental Cell Research 2017
L. Petecchia, C. Usai, M. Vassalli and P. Gavazzo
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Dynamics of Morphological Changes of Adherent Cultured Cells at a Range of Low Temperatures near Physiological Temperature (Time-Lapse Imaging by Digital Holographic Microscopy)
Cryobiology and Cryotechnology 2017
Hiroshi Ishiguro, Makoto Uemura and Kouhei Nagasaki
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Standardized Polyalthia longifolia leaf extract (PLME) inhibits cell proliferation and promotes apoptosis: The anti-cancer study with various microscopy methods
Biomedicine & Pharmacotherapy 2017
Soundararajan Vijayarathnaa, Yeng Chenb, Jagat R. Kanwarc and Sreenivasan Sasidharana
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Promising anticancer activity of Cyclotrichium niveum L. extracts through induction of both apoptosis and necrosis
Food and Chemical Toxicology 2017
Aysun Özdemira, Metin Yildiza, Fatma Sezer Senolb, Yaprak Dilber Şimaya, Burçin Ibişoglua, Alper Gokbulutc, Ilkay Erdogan Orhanb and Mustafa Ark
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Mannich Curcuminoids as Potent Anticancer Agents
Archiv der Pharmazie 2017
M. Gyuris, L. Hackler, L. Nagy, R. Alföldi, E. Rédei, A. Marton, T. Vellai, N. Faragó, B. Ózsvári, A. Hetényi, G. Tóth, P. Sipos, I. Kanizsai and L. Puskás
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Characterization of a transgenic mouse model exhibiting spontaneous lung adenocarcinomas with a metastatic phenotype
Hsuen-Wen Chang, Zih-Miao Lin, Min-Ju Wu, Li-Yu Wang, Yen-Hung Chow, Shih Sheng Jiang, Hui-Ju Ch’ang and Vincent HS Chang
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The “inherent vice” in the anti-angiogenic theory may cause the highly metastatic cancer to spread more aggressively
Scientific Reports 2017
Denian Wang, Chun Tan, Fei Xiao, Lan Zou, Lijun Wang, Yong’gang Wei, Hanshuo Yang, and Wei Zhang
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Supervised classification of etoposide-treated in vitro adherent cells based on noninvasive imaging morphology
Journal of Medical Imaging 2017
Anna Leida Mölder, Johan Persson, Zahra El-Schich, Silvester Czanner, Anette Gjörloff-Wingren
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A biophysical approach to quantify skeletal stem cells trans-differentiation as a model for the study of osteoporosis
Biophysical Chemistry 2017
L. Petecchia, F. Viti, F. Sbrana, M. Vassalli and P. Gavazzo
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Three-dimensional morphology of C6/36 cells infected by dengue virus: a study based on digital holographic microscopy
Journal of Southern Medical University 2017
Yu JH, Liu XL, Liu YJ, He XE, Hui Y, Zhang B, Zhu L and Zhao W
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Holography: The Usefulness of Digital Holographic Microscopy for Clinical Diagnostics
Holographic Materials and Optical Systems 2017
Zahra El-Schich, Sofia Kamlund, Birgit Janicke, Kersti Alm and Anette Gjörloff Wingren
Open access book chapter discussing the potential of holographic microscopy in clinical applications.
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Hydroxylated Fluorescent Dyes for Live-Cell Labeling: Synthesis, Spectra and Super-Resolution STED
Chemistry — A European Journal 2017
Alexey Butkevich, Vladimir Belov, Kirill Kolmakov, Viktor Sokolov, Heydar Shojaei, Sven Sidenstein, Dirk Kamin, Jessica Matthias, Rifka Vlijm, Johann Engelhardt and Stefan Hell

In sharp contrast to super-resolved fluorescence microscopes, HoloMonitor images and measures cells without using invasive dyes or reagents. Using HoloMonitor, the authors proved that cells stay alive and continue to divide after staining them with the improved super-resolution dyes.

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Investor note

The article is discussed in CEO commentary “Scale matters”, read it here.

Label-Free High Temporal Resolution Assessment of Cell Proliferation Using Digital Holographic Microscopy
Cytometry Part A 2017
Birgit Janicke, Andreas Kårsnäs, Peter Egelberg and Kersti Alm
The authors have developed a robust and label-free kinetic cell proliferation assay with high temporal resolution for adherent cells using HoloMonitor M4. Only two image processing settings were adjusted between cell lines, making the assay practical, user friendly, and free of user bias. In the recorded time-lapse image sequences, individual cells were automatically identified to provide detailed growth curves and growth rate data of cell number, confluence, and average cell volume. The results demonstrate how these parameters facilitate a deeper understanding of cell processes than what is achievable with current single-parameter and end-point methods.
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Investor note

The article is discussed in CEO commentary “The label-free promise”, read it here.

Applications of Label-Free, Quantitative Phase Holographic Imaging Cytometry to the Development of Multi-Specific Nanoscale Pharmaceutical Formulations
Cytometry Part A 2017
Ed Luther, Livia P. Mendes, Jiyai Pan, Daniel F. Costa and Vladimir P. Torchilin
A review of HoloMonitor applications:
  • tracking of Giant HeLa cells, which may be undergoing neosis, a process of tumor stem cell generation;
  • tracking the effects of cell cycle related toxic agents on cell lines;
  • using MicroRNAs to reverse the polarization state in macrophages to induce tumor cell killing
  • development of liposomal nanoformulations to overcome multi-drug resistance in ovarian cancer cells and
  • development of dual sensitive micelles to specifically target matrix metalloproteinase 2.
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Investor note

The article is discussed in CEO commentary “Fighting cancer with nanoparticles”, read it here.

Quantitative Phase-Contrast Imaging – A Potential Tool for Future Cancer Diagnostics
Cytometry Part A 2017
Anette Gjörloff-Wingren
Prof. Gjörloff-Wingren has used holographic microscopy in cancer related research for over a decade. Gjörloff-Wingren discuss the emerging field of quantitative phase imaging and the novel label-free capabilities that promise to improve and succeed invasive laborious photochemical procedures.
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Characterization of Dormant and Active Human Cancer Cells by Quantitative Phase Imaging
Cytometry Part A 2017
Peng Guo, Jing Huang and Marsha A. Moses
Based on HoloMonitor M4, the authors developed a novel in vitro method to characterize the angiogenic and non-angiogenic phenotypes of human osteosarcoma cells. Compared with traditional in vitro assays, the approach provides a non-invasive and low-cost method to assess a panel of cell morphology and cell behaviors. The authors were able to easily and accurately differentiate between the angiogenic and non-angiogenic phenotypes of KHOS cells by comparing five cellular parameters: cell area, cell thickness, cell migration, cell motility and cell motility speed.
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Investor note

The article is discussed in CEO commentary “No Blood, No Tumor”, read it here.

Loss of ERα induces amoeboid-like migration of breast cancer cells by downregulating vinculin
Nature Communications 2017
Yuan Gao, Zhaowei Wang, Qiang Hao, Weina Li, Yujin Xu, Juliang Zhang, Wangqian Zhang, Shuning Wang, Shuo Liu, Meng Li, Xiaochang Xue, Wei Zhang, Cun Zhang and Yingqi Zhang

“Oestrogen receptor alpha (ERα) is a well-known target of endocrine therapy for ERα-positive breast cancer. ERα-negative cells, which are enriched during endocrine therapy, are associated with metastatic relapse. Here we determine that loss of ERα in the invasive front and in lymph node metastasis in human breast cancer is significantly correlated with lymphatic metastasis. Using in vivo and in vitro experiments, we demonstrate that ERα inhibits breast cancer metastasis. Furthermore, we find that ERα is a novel regulator of vinculin expression in breast cancer. Notably, ERα suppresses the amoeboid-like movement of breast cancer cells by upregulating vinculin in 3D matrix, which in turn promotes cell–cell and cell–matrix adhesion and inhibits the formation of amoeboid-like protrusions. A positive association between ERα and vinculin expression is found in human breast cancer tissues. The results show that ERα inhibits breast cancer metastasis and suggest that ERα suppresses cell amoeboid-like movement by upregulating vinculin.”

The authors tracked and analyzed the migration of different MCF-7 cell populations in a 3D matrix. In addition to measuring the cell migration speed with HoloMonitor, the following five phenotypes were observed: ‘stable adhesion’, ‘unstable lamellipodia’, ‘bleb’, ‘stable bleb’ and ‘unstable pseudopod’.

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Investor note

Tamoxifen is routinely used to prevent the most common type of breast cancer by blocking the effects of estrogen. Using HoloMonitor scientists have gained valuable insight into why Tamoxifen increases the risk of other types of breast cancer. Read more about hormone treatment of breast cancer here.

In situ morphological assessment of apoptosis induced by Phaleria macrocarpa (Boerl.) fruit ethyl acetate fraction (PMEAF) in MDA-MB-231 cells by microscopy observation
Biomedicine & Pharmacotherapy 2017
Nowroji Kavithaa, Yeng Chenb, Jagat R. Kanwarc and Sreenivasan Sasidharan
HoloMonitor imaging results of living MDA-MB-231 human breast cancer cells after treated with IC50 concentration of PMEAF for an incubation time of (a) untreated, (b) 6, (c) 12 and (d) 24 h.

Phaleria macrocarpa is a well-known medicinal plant and have been extensively used as traditional medicine for ages in treatment of various diseases.

The purpose of the study was to determine the cytotoxic effect of P. macrocarpa fruit ethyl acetate fraction (PMEAF) by using various conventional and modern micro­scopy techniques. The results indicate that PMEAF is a potential anticancer agent and a candidate for developing more potent anticancer drugs.

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Investor note

Many anti-cancer drugs that are in use today originate from traditional medicinal plants.


Hepatitis B virus X induces inflammation and cancer in mice liver through dysregulation of cytoskeletal remodeling and lipid metabolism
Oncotarget 2016
Zhongwei Xu, et al.

It has previously been shown that the hepatitis B virus gene HBx induces liver cancer in mice. The results expand these findings by providing evidence of how the HBx gene induces liver cancer.

To study the effect of HBx gene the movement of human liver cells (Huh-7) was assessed using the HoloMonitor M4 time-lapse imaging cytometer, 36 h after introducing the HBx gene. Images were captured once every 5 min during 24 h. The mean migration distance per cell was obtained using the HoloMonitor M4 cell tracking software.

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Investor note

Liver cancer is the fifth most common cancer worldwide, and the fourth most common cause of cancer-related death. The main cause of liver cancer is a chronic hepatitis B or C virus infection.

Non-invasive, quantitative assessment of the morphology of γ-irradiated human mesenchymal stem cells and periosteal cells using digital holographic microscopy
International Journal of Radiation Biology 2016
T. Kawase, K. Okuda, M. Nagata, M. Tsuchimochi, H. Yoshie and K. Nakata
To assure the quality of cells to be used in cell therapy, the applicability of HoloMonitor technology is examined for non-invasive, quantitative assessment of changes in cell morphology. Instead of DNA damage markers, the authors suggest that morphological parameters could be a useful and more stable marker of stem cell quality.
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Investor note

Stem cells are cells which develop into specific cell types. Scientists hope to be able to treat a range of currently incurable diseases by transplanting cells which has been created from stem cells in a cell laboratory.
Lipid deregulation in UV irradiated skin cells: Role of 25-hydroxy­cholesterol in keratinocyte differentiation during photoaging
The Journal of Steroid Biochemistry and Molecular Biology 2016
E. Olivier, M. Dutot, A. Regazzetti, D. Dargère, N. Auzeil, O. Laprévote and P. Rat

Skin photoaging due to UV irradiation is a degenerative process that appears more and more as a growing concern. Lipids, including oxysterols, are involved in degenerative processes; as skin cells contain various lipids, the aim of our study was to evaluate first, changes in keratinocyte lipid levels induced by UV exposure and second, cellular effects of oxysterols in cell morphology and several hallmarks of keratinocyte differentiation.

Using HoloMonitor and confocal microscopy analysis, the authors highlighted cell thickening and cytoskeletal damage after incubation of keratino­cytes with 25-hydroxycholesterol.

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Investor note

By combining HoloMonitor and fluorescence microscopy the authors investigated how UV-light and oxidized cholesterol (oxysterols) affect the aging of skin cells (keratinocytes). Skin aging is a growing concern. One in eight people in the world are today 60 years old or older.

Keratinocytes are the most common cells in the outermost layer of the skin.
Lipids are a group of naturally occurring molecules that include fats, waxes and sterols.
Differentiation is the process by which a less specialized cell becomes a more specialized cell type.

Ca2+ signals, cell membrane disintegration, and activation of TMEM16F during necroptosis
Cellular and Molecular Life Sciences 2016
J. Ousingsawat, I. Cabrita, P. Wanitchakool, L. Sirianant, S. Krautwald, A. Linkermann, R. Schreiber and K. Kunzelmann

It is commonly accepted that cells swell during necroptosis. However, proper cell volume measurements are still missing. Using Holo­Monitor M4 the authors non-invasively measured the cell volume changes associated with necroptosis. The results were confirmed by flow cytometry measurements.

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Investor note

Necroptosis is a controlled form of cell death. Before necroptosis was discovered around 2000, it was believed that cells die by either being killed (necrosis) or by committing suicide (apoptosis). The discovery of necroptosis indicates that cell death is far more complicated than initially believed.

The understanding of cell death is important as it may lead to cancer treatments that precisely kill the cancer cells while sparing the normal cells, resulting in treatments with fewer side effects.

Relationship between TMEM16A/anoctamin 1 and LRRC8A
European Journal of Physiology 2016
R. Benedetto, L. Sirianant, I. Pankonien, P. Wanitchakool, J. Ousingsawat, I. Cabrita, R. Schreiber, M. Amaral and K. Kunzelmann

Using time courses the authors have studied hypotonic cell swelling and re-shrinking. Cell height measured by Holo­Monitor M4 in HEK293 cells indicates cell swelling within seconds and completed regulatory volume decrease (RVD) within less than 2 min. The authors analyzed hypo-induced changes in cell height rather than cell volume, as an increase in cell height is not compromised by basal adhesions or cell/cell contacts.

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Investor note

Hypotonic is when there is a lower concentration outside a cell than inside. As water rushes into the cell to compensate for the concentration difference the cell swells. The authors have used HoloMonitor’s unique ability to continuously measure cell height and volume to study how cells respond to various concentration differences. In the above image it can be seen that cell height rapidly increase when cells are exposed to a concentration difference and thereafter slow decrease in height to abruptly drop back to normal when the concentration difference is removed. It can also be seen that the swelling response is greater at higher concentration differences.

Reversal of chemoresistance in ovarian cancer by co-delivery of a P-glycoprotein inhibitor and paclitaxel in a liposomal platform
Molecular Cancer Therapeutics 2016
Yilin Zhang, Shravan Kumar Sriraman, Hilary A Kenny, Ed Luther, Vladimir Torchilin and Ernst Lengyel
HoloMonitor images of induced mitotic arrest, as evidenced by increased cellular thickness (red) and larger cell volume.

Ovarian cancer causes more deaths than any other cancer of the female reproductive system. The disease recurs in 80% of cases because the tumor cells develop drug resistance.

The label-free quantitative technique facilitated by HoloMonitor allowed scientists from University of Chicago and Northeastern University in Boston to follow the development of mitotic arrest in real time.

It permitted the scientists to conclude that tariquidar treatment of resistant cancer cells re-sensitized the cells to paclitaxel, allowing the drug to again stop the cancer cells from multiplying by inducing almost complete mitotic arrest.

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Mixed Nanosized Polymeric Micelles as Promoter of Doxorubicin and miRNA-34a Co-Delivery Triggered by Dual Stimuli in Tumor Tissue
Small 2016
Guseppina Salzano, Daniel F. Costa, Can Sarisozen, Ed Luther, George Mattheolabakis, Pooja P. Dhargalkar, and Vladimir Torchilin
4D plots (top) and cell tracks (bottom).

“For the analysis in a 2D migratory model, cells were imaged with a HoloMonitor M4 system. The auto­mated stage allowed multiple collection of fields of view over time. Images were segmented and a list of features, including cell volume, thickness, and area were obtained, along with cell positional information. Values for the migration and motility of the cells were calculated and presented as cell tracks with the HoloMonitor software.

Methods for producing 4D plot were developed. Images from the time course were exported to ImageJ and treated as an image stack, creating a volume rendering of the cellular motion over time of analysis. The brightness threshold was adjusted to view all cells within the imaging area or only the thicker cells, a feature that allows identification of mitosis, apoptosis, and transition between mesenchymal and amoeboid cellular morphologies.”

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Label-Free Optical Biosensors for Monitoring Cellular Processes and Cytotoxic Agents at Interfaces Using Guided Modes and Advanced Phase-Contrast Imaging Techniques
Advanced Sciences and Technologies for Security Applications 2016
Inna Székács, Robert Horvath and András Székács
Cells under toxic effect visualized by HoloMonitor M4.
“Quantitative visualization of average cell thickness and area, calculated using the HoloMonitor M4 software, indicated effects on the cells in close correlation with those determined in colorimetric cytotoxicity tests, indicating, besides the toxicological significance, the utility of the digital holographic monitoring technique in toxicology.”
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Biophysical characteristics of proteins and living cells exposed to the green tea polyphenol epigallocatechin-3-gallate
European Biophysics Journal 2016
Beatrix Peter, Szilvia Bosze and Robert Horvath
HoloMonitor-based methods and other methods to assess cell adhesion, motility, migration, stiffness, apoptosis and proliferation exposed to green tea polyphenol epigallocatechin-3-gallate are reviewed.
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Ion channels in regulated cell death
Cellular and Molecular Life Sciences 2016
K. Kunzelmann
Cell appearances during necroptotic cell death of NIH3T3 cells imaged by HoloMonitor M4.
Despite the rather different morphologic appearances of apoptosis and necroptosis, both mechanisms of cell death require the transport of ions, organic osmolytes, and water. The contribution of these transport proteins and underlying mechanisms are summarized in this review.
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Shielding of the Geomagnetic Field Alters Actin Assembly and Inhibits Cell Motility in Human Neuroblastoma Cells
Scientific Reports 2016
W-C. Mo, Z-J. Zhang, D-L. Wang, Y. Liu, P. Bartlett and R-Q. He
Earth’s magnetic field is a natural part of our environment. Using HoloMonitor M4, the authors show that the elimination of Earth’s magnetic field affects cell behavior. According to the authors, the findings suggest that magnetic field elimination may be used to prevent tumor progression in future clinical applications. The findings also provide clues to which health risks astronauts are exposed to during deep space explorations, where no magnetic field is present.
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Pseudomonas aeruginosa N-3-oxo-dodecanoyl-homoserine Lactone Elicits Changes in Cell Volume, Morphology, and AQP9 Characteristics in Macrophages
Frontiers in Cellular and Infection Microbiology 2016
A. Holm, K-E. Magnusson and E. Vikström

Pseudomonas aeruginosa is one of the antibiotic resistant bacteria types that cause hospital-acquired infections. In combination with other imaging techniques, the authors used HoloMonitor M4 to study how these bacteria interact with the immune system.

Pseudomonas aeruginosa infection is a serious problem in patients hospitalized with cancer, cystic fibrosis and burns. The case fatality rate in these patients is exceedingly high. Research for the discovery of new antibiotics and drugs against P. aeruginosa is therefore very much needed.

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The Curcumin Analog C-150, Influencing NF-κB, UPR and Akt/Notch Pathways Has Potent Anticancer Activity In Vitro and In Vivo
L. Hackler et al.
Holographic microscopic analysis of C-150 induced cell death using HoloMonitor M3

C-150 – a Mannich-type curcumin derivative – exhibited pronounced cytotoxic effects against eight glioma cell lines at micromolar concentrations. Inhibition of cell pro­lifera­tion by C-150 was mediated by affecting multiple targets as confirmed at transcription and protein level. C-150 effectively reduced the transcription activation of NFkB, inhibited PKC-alpha which are constitutively over-expressed in glioblastoma.

Glioblastoma is the most common and most aggressive cancer originating within the brain.

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From immobilized cells to motile cells on a bed-of-nails: effects of vertical nanowire array density on cell behavior
Scientific Reports 2015
H. Persson, Z. Li, J. O. Tegenfeldt, S. Oredsson and C. N. Prinz

During recent years, vertical nanowire arrays have received increasing atten­tion for their possible use in life sciences, as electrodes, biosensors, as well as for axonal guidance, cell injections and anti-bacterial proper­ties. The rapidly expanding number of nanowire applications calls for a better under­standing of the inter­actions between cells and nanowires.

Using HoloMonitor the researchers investigated the effects of nanowire density on cell movement, division and morphology. The results provide guidelines to minimize cellular stress on nanowire arrays. Additionally, the findings show that cell behavior can be controlled by adjusting nanowire density, which may have applications in drug development.

Article Image: Path of L929 cells migrating for 20 h on the different substrates
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Enhanced Cytotoxicity of Folic Acid-Targeted Liposomes Co-Loaded with C6 Ceramide and Doxorubicin: In Vitro Evaluation on HeLa, A2780-ADR and H69-AR Cells
Molecular Pharmaceutics 2015
S. K. Sriraman, J. Pan, C. Sarisozen, E. Luther and V. Torchilin
Article figure show showing drop in cell volume

Current research in cancer therapy is shifting towards the use of combinational drug-treatment regimens. However, the efficient delivery of drug combinations is governed by a number of complex factors in the clinical setting.

The development of a novel folic acid-targeted liposomal formulation, simultaneously co-loaded with C6 ceramide and doxorubicin is reported. FA-(C6+Dox)-LP were able to significantly enhance the induction of apoptotic events in HeLa cell mono­layers as compared to the other treatment groups.

Using HoloMonitor M4, it was found that FA-(C6+Dox)-LP treated HeLa cells underwent rapid progression to apoptosis (cell suicide) after 21 hours, as evidenced by a drastic drop in cell area after loss of cell membrane integrity.

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Targeted tumor therapy by Rubia tinctorum L.: analytical character­ization of hydroxyan­thraquinones and investigation of their selective cytotoxic, adhesion and migration modulator effects on melanoma cell lines
Cancer Cell International 2015
E. Lajkó, P. Bányai, Z. Zámbó, L. Kursinszki, É. Szőke and L. Kőhidai

The effects of alizarin, purpurin and an aqueous extract from transformed hairy root culture of Rubia tinctorum L. were examined on (1) cell proliferation, (2) apoptosis, (3) cell adhesion/morphology and (4) migration (chemotaxis, chemokinesis) of human melanoma cell lines (A2058, HT168-M1) and human fibroblast cells (MRC-5), as well as (5) the aqueous extract was analytically characterized.

All tested substances elicited chemo­repellent effect in melanoma cells (malignant skin cancer cells), while in MRC-5 fibroblasts, only the alizarin exhibited such a repellent character. Indices of chemo­kinesis measured by Holo­Monitor M4 (migration, migration directness, motility and motility speed) were signifi­cantly enhanced by alizarin and purpurin as well, while morphometric changes were weak in the two melanoma cell lines.

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Cathelicidins positively regulate pancreatic β-cell functions
The FASEB Journal 2015
J. Sun, M. Xu, H. Ortsäter, E. Lundeberg, L. Juntti-Berggren, Y. Q. Chen, J. Z. Haeggström, G. H. Gudmundsson, J. Diana and B. Agerberth
Article Image: Cell density after various treatments, imaged using HoloMonitor M4
Cells were seeded in a 6-well plate and allowed to adhere in RPMI 1640 medium overnight. After washing with PBS, complete media containing 0.01 mg/L scrambled rCRAMP, 0.1 mg/L scrambled rCRAMP, and 0.01 mg/L rCRAMP were added into wells. HoloMonitor M4 was used to assess the cell density during cell proliferation. An image was taken for each well per day up to 4 days.
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Macrophage Polarization and the Effect of MicroRNA-155 Administered in Water-in-Oil-in-Water Multiple Emulsion Formulations
Journal of Clinical & Cellular Immunology 2015
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Oka A, Talekar M, Ouyang Q, Luther E and Amiji M
The tumor microenvironment is composed of range of cells including macro­phages that participate in sustaining tumor growth and invasion. Repolarized macrophages were co-cultured with SKOV3 ovarian cancer cells to assess the effect on macrophage morphology, motility and apoptosis of cancer cells. Holographic assessment using HoloMonitor M4 of the co-cultured cells in real-time showed differences in motility and morphology of macrophages with miR-155 ME treated cells showing greater cellular interaction between the two phenotypes.
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Poloxamer bioadhesive hydrogel for buccal drug delivery: Cytotoxicity and trans-epithelial permeability evaluations using TR146 human buccal epithelial cell line
International Journal of Pharmaceutics 2015
N. Zenga, N. Mignet, G. Dumortier, E. Olivier, J. Seguin, M. Maury, D. Scherman, P. Rat and V. Boudy
A bioadhesive hydrogel specially developed for buccal administration was investigated by studying interactions with TR146 human buccal epithelium cells. The assessment of cell viability (MTT, Alamar Blue), membrane integrity (Neutral Red), and apoptosis assay (Hoechst 33342), were performed and associated to HoloMonitor M3 analysis. The agreement with the cell viability results shows various advantages of the HoloMonitor technique: less preparation time compared to cell staining assays, rapidity of detection and robustness for formulation screening.
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Incubator proof miniaturized HoloMonitor to in situ monitor cancer cells exposed to green tea polyphenol and preosteoblast cells adhering on nanostructured titanate surfaces: validity of the measured parameters and their corrections
J. Biomed. Opt 2015
Beatrix Peter, Judit Nador, Krisztina Juhasz, Agnes Dobos, Laszlo Korosi, Inna Székács, Daniel Patko and Robert Horvath
HoloMonitor M4 was used to observe cancer cell motility, motility speed, and migration in the presence of the green tea polyphenol, epigallocatechin gallate, as well as to monitor the adhesion of preosteoblast cells on nanostructured titanate coatings, relevant for biomedical applications.
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Reversal of Chemoresistance in Ovarian Cancer by Co-Delivery of MDR Inhibitors and Paclitaxel Using a Targeted Liposomal Platform
The FASEB Journal 2015
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Shravan Kumar Sriraman, Yilin Zhang, Ernst Lengyel and Vladimir Torchilin
Co-loaded liposomes were able to effectively reverse chemoresistance in a variety of drug-resistant ovarian cancers. The cellular effects of the formulations were evaluated using HoloMonitor M4.
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Interfacing antibody-based microarrays and digital holography enables label-free detection for loss of cell volume
Future Science OA 2015
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Zahra El-Schich, Emmy Nilsson, Anna S Gerdtsson, Christer Wingren and Anette Gjörloff Wingren
The article provides proof of concept for using holographic microscopy combined with antibody-based microarray technology for detecting morphological changes in captured cells.
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Induction of morphological changes in death-induced cancer cells monitored by holographic microscopy
Journal of Structural Biology 2015
Zahra El-Schich, Anna Mölder, Helena Tassidis, Pirkko Härkönen, Maria Falck Miniotis and Anette Gjörloff Wingren
Using HoloMonitor M3, the authors show that holographic microscopy allows label-free and completely non-invasive morphological measurements of cell growth, viability and death.
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Quantitative Stain-Free and Continuous Multimodal Monitoring of Wound Healing In Vitro with Digital Holographic Microscopy
D. Bettenworth et al.
In this study, it is shown that holographic microscopy enables continuous, stain-free monitoring of intestinal epithelial wound healing in vitro and to provide simultaneous quantification of key cellular characteristics such as cell volume, cell thickness, dry mass and cell density which may help to characterize therapeutic effects of potential drug candidates.
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Live-cell mass profiling: an emerging approach in quantitative biophysics
Nature Methods 2014
Thomas A Zangle and Michael A Teitell
The authors discuss how live-cell mass profiling can be achieved by using holographic microscopy and how this can revolutionize our understanding of cellular physiology and growth.
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Combination of unsaturated fatty acids and ionizing radiation on human glioma cells: cellular, biochemical and gene expression analysis
Lipids in Health and Disease 2014
Otilia Antal, László Hackler, Junhui Shen, Imola Mán, Katalin Hideghéty, Klára Kitajka and László G Puskás
HoloMonitor M3 was used to assess the increased death of glioma (brain tumor) cells when combining irradiation with various unsaturated fatty acid treatments.
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Digital Holographic Microscopy for Non-Invasive Monitoring of Cell Cycle Arrest in L929 Cells
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Maria Falck Miniotis, Anthonny Mukwaya and Anette Gjörloff Wingren
The study show that holographic microscopy is able to monitor drug-induced cell cycle arrest in cultured cells and thus provide a non-disruptive alternative to flow cytometry.
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Edaravone Protects against Methylglyoxal-Induced Barrier Damage in Human Brain Endothelial Cells
Andrea E. Tóth et al
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The authors use HoloMonitor M3 to study morphological alterations induced in human brain endothelial cells (hCMEC/D3) by treatment with methylglyoxal and co-treatment with edaravone for 4 hours. Methylglyoxal triggers carbonyl stress and activates a series of inflammatory responses leading to accelerated vascular damage. Edaravone is the active substance of a Japanese medicine, which aids neurological recovery following acute brain ischemia and subsequent cerebral infarction. The aim of the study was to test whether edaravone can exert a protective effect on the barrier properties of human brain endothelial cells (hCMEC/D3 cell line) treated with methylglyoxal.
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The role of PI3K/AKT-related PIP5K1α and the discovery of its selective inhibitor for treatment of advanced prostate cancer
PNAS 2014
J. Semenas et al.
HoloMonitor M3 was used to show which treatments that lead to changed morphology and reduced size of PC-3 cells.
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Environmental and Toxicological Impacts of Glyphosate with Its Formulating Adjuvant
International Journal of Agricultural, Biosystems Science and Engineering 2014
I. Székács et al.
The article describes a study of the health effects of the well-known, widely used herbicide Roundup. In the study, the HoloMonitor M4 was used to assess toxic effects to animal cells after exposure to Roundup.
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Prevention and treatment of colon cancer by peroral administration of HAMLET
Gut 2013
M. Puthia, P. Storm, A. Nadeem, S. Hsiung, C. Svanborg
Using the HoloMonitor M2, the authors show that cell thickness and adherent surface area change drama­tically after HAMLET treatment. HAMLET is a human α-lactalbumin made lethal to tumor cells.
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Lipids as Tumoricidal Components of Human α-Lactalbumin Made Lethal to Tumor Cells (HAMLET): UNIQUE AND SHARED EFFECTS ON SIGNALING AND DEATH
J. of Biological Chemistry 2013
J. C. S. Ho, P. Storm, A. Rydström, B. Bowen, F. Alsin, L. Sullivan, I. Ambite, K. H. Mok, T. Northen and C. Svanborg
The authors used the HoloMonitor M3 to observe morphological differences on cells over time, treated with either Hamlet or various lipids associated with HAMLET.
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A Unifying Mechanism for Cancer Cell Death through Ion Channel Activation by HAMLET
P. Storm, T. K. Klausen, M. Trulsson, J. C. S Ho, M. Dosnon, T. Westergren, Y. Chao, A. Rydström, H. Yang, S. F. Pedersen and C. Svanborg
Changes in cell morphology was studied using HoloMonitor technology.
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Fibroblasts Cultured on Nanowires Exhibit Low Motility, Impaired Cell Division, and DNA Damage
Small 2013
H. Persson, C. Købler, K. Mølhave, L. Samuelson, J. O. Tegenfeldt, S. Oredsson and C. N. Prinz
Fibroblast behaviour on vertical nanowire arrays was investigated using the Holo­Monitor M3. It was shown that cell motility and proliferation rate are reduced on nanowires. Fibroblasts cultured on long nanowires exhibit failed cell division, DNA damage, increased ROS content and respiration.
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Cells and Holograms — Holograms and Digital Holographic Microscopy as a Tool to Study the Morphology of Living Cells
Holography — Basic Principles and Contemporary Applications 2013
K. Alm, Z. El-Schich, M. Falck Miniotis, A. Gjörloff Wingren, B. Janicke and S. Oredsson
A dying unstained L929 mouse fibroblast cell, imaged every four minutes after etoposide (50µM) treatment. The arrows point to a cell, which starts its death process by contracting slightly. Optically, the cell content becomes thin and finally the cell remnants start to dissolve.
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2012 and earlier

Low Resolution Solution Structure of HAMLET and the Importance of Its Alpha-Domains in Tumoricidal Activity
J. C. S. Ho, A. Rydström, M. S. S. Manimekalai, C. Svanborg and G. Grüber
The authors use the HoloMonitor M3 to detect morphological changes in A549 lung carcinoma cells treated with HAMLET, alpha1 peptide+oleate, alpha2 peptide+oleate and beta peptide+oleate.
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HAMLET: functional properties and therapeutic potential
Future Medicine 2012
J. C. S. Ho, A. Rydström, M. Trulsson, J. Bålfors, P. Storm, M. Puthia, A. Nadeem and C. Svanborg
The authors use HoloMonitor M3 to study morphological kinetics in lung carcinoma cells exposed to HAMLET.
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Dermatan Sulfate is involved in the tumorigenic properties of esophagus squamos cell carcinom
Cancer Research 2012
M. A. Thelin, K. J. Svensson, X. Shi et al.
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Digital Holography and Cell Studies in Holography
Research and Technologies 2011
K. Alm, H. Cirenajwis, L. Gisselsson, A. Gjörloff Wingren, B. Janicke, A. Mölder, S. Oredsson, J. Persson
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HAMLET Binding to α-Actinin Facilitates Tumor Cell Detachment
M. Trulsson, H. Yu, L. Gisselsson, Y. Chao, A. Urbano, S. Aits, A-K. Mossberg, C. Svanborg
Trulsson et al. used the label-free cell morphology analysis capabilities of the HoloMonitor to follow the cell death process of A549 lung cancer cells treated with HAMLET (right). HAMLET is a milk protein-lipid complex which is both tumoricidal and triggers cell detachment both in vivo and in vitro. HAMLET treatment therefore caused cells to round up and become less optically dense, which was clearly quantified by the HoloMonitor.
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Reduction of the putative CD44+CD24 breast cancer stem cell population by targeting the polyamine metabolic pathway with PG11047
Anticancer Drugs 2010
Cirenajwis et al.
Cirenajwis and colleagues used the label-free live cell imaging capabilities of the HoloMonitor to study the movement of JIMT-1 breast cancer cells. They showed that treatment with the ornithine analogue difluoromethylor-nithine (DFMO) increased cell motility, while treatment with the polyamine analogue PG-11047 decreased cell motility.
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Generation of trisomies in cancer cells by multipolar mitosis and incomplete cytokinesis
PNAS 2010
David Gisselsson Nord et al.
In collaboration with PHI, Gisselsson and colleagues studied cell division in short-term cultures established from fibroblasts and primary tumors. The cultures were followed for up to ten days using Holo­Monitor M2. The researchers showed that abnormal cell divisions can cause an abnormal number of chromosomes in cancer cells.
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More about David Gisselsson Nord

Holographic time-lapse series showing a tripolar metaphase (green broken lines; t = 0) followed by tripolar ana-telophase (t = 14–36 min). Cytokinesis is initiated along two cleavage furrows in this cell division (t = 70 min), but one of the cleavage furrows regresses and only two daughter cells are formed (t = 306 min), of which the larger is binucleate as evidenced by two clusters of nucleoli. The larger cell (t = 3,192 min) enters mitosis (red broken lines), forming a single mitotic plate and divides into two daughters (t = 3,586 min). Daughter cells from both cell divisions remained without evidence of cell death or degeneration throughout the observation time.

Digital Holography and Cell Motility
Microscopy: Science, Technology, Applications and Education 2010
J. Persson, A. Mölder, S-G. Pettersson, K. Alm
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Immunomodulatory nanoparticles as adjuvants and allergen-delivery system to human dendritic cells: Implications for specific immunotherapy
Vaccine 2010
S. Broos, K. Lundberg, T. Akagi, K. Kadowaki, M. Akashi, L. Greiff, C. A. Borrebaeck, M. Lindstedt
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Digital holographic microscopy — innovative and non-destructive analysis of living cells
Microscopy: Science, Technology, Applications and Education 2010
Z. El-Schish, A. Mölder, M. Sebesta, L. Gisselsson, K. Alm, A. Gjörloff Wingren
3D phase shift image of antibody-targeted T leukemia cells, taken from the article.
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Non-invasive, label-free cell counting and quantitative analysis of adherent cells using digital holography
J. of Microscopy 2008
Anna Mölder et al.
Mölder et al. show that cell counting using the HoloMonitor yields results equivalent to counting in a hemo­cytometer. Using the HoloMonitor to measure cell confluence yields results comparable to cell counting. Instead of cell number or confluence, optical density (cell dry mass) can be used as a cell counting parameter. The diagram shows manual and HoloMonitor-assisted cell counting as well as confluence of L929 mouse fibroblast cells.
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A hardware acceleration platform for digital holographic imaging
Journal of VLSI Signal Processing Systems 2008
T. Lenart, M. Gustafsson, V. Öwall
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Object characterization with refractometric digital Fourier holography
Optics Letters 2005
M. Sebesta, M. Gustafsson
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Refractometry of microscopic objects with digital holography
Applied Optics 2004
M. Gustafsson, M. Sebesta
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High-resolution digital transmission microscopy — a Fourier holography approach
Optics and Lasers in Engineering 2004
M. Gustafsson, M. Sebesta, B. Bengtsson, S.G. Pettersson, P. Egelberg, T. Lenart
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Conference papers & posters

Cystatin C decreases proliferation of melanoma cells by affecting mitosis length
AACR Annual Meeting 2017
Hanna Wallin, Kersti Alm and Magnus Abrahamson
Analysis using HoloMonitor revealed qualitative differences in the normal behavior of the A375 cells under study as a result of cystatin C addition. The motility of the cells was significantly affected. The mitosis phase was considerably prolonged in cells grown in cystatin C containing medium, indicating that the proliferation-inhibiting effect of the protease inhibitor may be through its direct action on proteolytic processes in the mitosis phase.
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Development of a 4-sample version of the Kolmogorov-Smirnov test for evaluating the temporal physiology of cells treated with test compounds in a label-free, high content, platform for quantitative analysis of adherent cell-culture models
AACR Annual Meeting 2017
Ed Luther, Livia Mendes, Jiayi Pan, Daniel Costa, Elena Holden and Vladimir Torchilin
4-dimensional plots of HoloMonitor time-lapse data were developed to assist in the visualization of the effects of pharmaceutical compounds under development.
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Impedance-based Analysis and Holographic Phase Imaging of the GnRH-III-based Drug-targeting in Melanoma Cells
3rd Conference on Impedance-Based Cellular Assays 2016
E. Lajkó, S. Spring, O. Láng, S. Ingebrandt, G. Mező and L. Kőhidai
HoloMonitor M4 was used for the morphometry and migration studies.
View abstract…
A label-free, high content, moderate throughput analytical platform for quantitative kinetic analysis of cell behavior upon drug activation based on the Kolmogorov-Smirnov test
AACR Engineering and Physics Applied to Oncology 2016
Ed Luther, Livia Mendes, Jiayi Pan, Daniel Costa, Elena Holden and Vladimir Torchilin
“Our objective is to develop multi-functional nanotechnology-based antitumor drug delivery systems for improving efficacy of treatments and reducing undesirable side effects. The essential part of this process is the development of un-biased quantitative analytical techniques. We are reporting a successful validation of a very high content, medium throughput system in multi-well plates, employing the newly developed holographic imaging cytometer HoloMonitor® M4 for label-free time-lapse cellular analysis.”
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Transcription regulation of chemokine receptor CXCR4 by nuclear respiratory factor 1 (NRF1) controls estrogen-induced malignant transformation of breast epithelial cells to breast cancer stem cells
AACR Annual Meeting 2016
J. K. Das and D. Roy
Chemokine receptor CXCR4 is involved in the maintenance of stemness property of stem cells, aging and metastasis of cancer. NRF1 is one of the major transcription factors that controls transcription of CXCR4. In this study, the authors have investigated whether transcription regulation of CXCR4 by NRF1 controls estrogen-induced malignant transformation of breast epithelial cells to breast cancer stem cells.
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HoloMonitor M4: holographic imaging cytometer for real-time kinetic label-free live-cell analysis of adherent cells
Proc. SPIE, Quantitative Phase Imaging II 2016
M. Sebesta, P. Egelberg, A. Langberg, J-H Lindskov, K. Alm and B. Janicke
HoloMonitor M4 is designed to provide long-term observations in the incubator environment
Live-cell imaging enables studying dynamic cellular processes that cannot be visualized in fixed-cell assays. An increasing number of scientists in academia and the pharma­ceutical industry are choosing live-cell analysis over or in addition to traditional fixed-cell assays. We have developed a time-lapse label-free imaging cytometer – HoloMonitor M4.
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Water homeostasis in infection and inflammation – Quorum sensing AQP9
ASCB Annual Meeting 2015
A. Holm, K-E. Magnusson and E. Vikström
Poster Image: Representative images of the time-lapse sequence used to quantify cell volume after treatment

Macrophages are an important part of the immune system. They are activated by molecules produced by infecting bacteria. Activation induces macrophages to elevate their membrane content through water specific gates. These aquaporins allow water to enter the cells and make them swell.

This poster by researchers at Linköping University in Sweden shows this increase of aquaporins after acti­vation by bacterial molecules. Using Holo­Monitor M4, morphology analysis of activated macrophages visualizes and quantifies the increase of cell volume, following the increased number of aquaporins in the cell membrane.

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Comparison of the effects of pharmaceutical compounds on tumor cells in 2D and 3D in vitro models using label-free, quantitative 4 dimensional holographic imaging
AACR-NCI-EORTC Inter. Conf. on Molecular Targets and Cancer Therapeutics 2015
E. Luther, G. Salzano, S. K. Sriraman, D. Costa and V. P. Torchilin
Poster Image: As the complexity of the formulations increases, there is a decrease in the high optical thickness and a corre­spond­ing increase in the low optical thick­ness consistent with increasing cell death.
We developed a novel 4D holographic imaging method using Holo­Monitor M4 and Image J. Here, we present methods for comparing traditional 2D and 3D in vitro models. Our example of HT1080 cells treated with dox clearly shows the superiority of the 3D model, an important step in developing assays that better emulate multi-dimensional biological processes and offer the possibility of evaluating effects of drugs at lower cost and experimental complexity than those of in vivo assays.
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Four dimensional quantitative label-free holographic imaging of the cell cycle in tumor cell lines
AACR Annual Meeting 2015
Ed Luther, Jeffrey Agar and Mansoor Amiji
Poster Image: Seeding of a giant HeLa cell
Label free imaging and analysis of cells using only their physical characteristics is desirable in pharma­ceutical studies because it eliminates the effects of labeling compounds on the cells under study. We used a newly developed holographic imaging system to monitor cell-cycle effects in long term cell cultures.
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Characterization of macrophage behavior by 4-dimensional label free quantitative holographic imaging
AACR Annual Meeting 2015
Ed Luther, Meghna Talekar, Adwait Oka, Qijun Ouyang and Mansoor Amiji
The tumor microenvironment consists of several cell types including cancer cells and stromal cells (fibroblasts and inflammatory cells) embedded in extracellular matrix. Among the inflammatory cells, macrophages are critical mediators of tumorigenesis. Their behavior and activation is controlled by a large number of chemo-stimulants, and are broadly classified into two polarization groups, M1 (classically activated macro­phages), that enable the release of cytokines and chemokines that induce tumor suppression and M2 (alternatively activated macrophages), that induce tissue repair and angiogenesis. In the tumor micro­environ­ment this translates into tumor promotion. We employed a newly developed label free holographic time lapse imaging system to characterize the morphology and motility of macrophages in response to stimulants and tumor cells in 4-dimensional models of the tumor microenvironment.
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Modeling and exploration of a reconfigurable architecture for digital holographic imaging
IEEE International Symposium on Circuits and Systems 2008
T. Lenart, H. Svensson, V. Öwall
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Docteral thesis

Characterization of the novel pericyte receptors S1PR3 and PTGER2
Faculty of Biosciences, Heidelberg University 2017
Doctoral thesis by Tina Heumann
Morphological changes were evaluated by comparing time-plots of cell volume and thickness, created by HoloMonitor M4.
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Proteins, polymers and living cells on titanium oxide-based nanostructured coatings: exploitation of label-free optical sensing methods
Doctoral School of Molecular- and Nanotechnologies, University of Pannonia 2016
Doctoral thesis by Judit Nádor
The HoloMonitor M4 was used to monitor living cells (human embryonic kidney cells and preosteoblasts) on nanostructured coatings in a novel cuvette configuration.
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Modulation of tight junctions by pepties to increase drug penetration across biological barriers
Department of Pharmaceutical Technology, University of Szeged 2016
Doctoral thesis by Alexandra Bocsik
Morphological changes in peptide treated Caco-2 epithelial cells were analyzed using HoloMonitor technology.
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Combination of unsaturated fatty acids and ionizing radiation on human glioma cells: cellular, biochemical and gene expression analysis
Department of Pharmaceutical Technology, University of Szeged 2016
Doctoral thesis by Otilia Antal
HoloMonitor M3 was used to assess the increased death of glioma (brain tumor) cells when combining irradiation with various unsaturated fatty acid treatments.
Novel imaging technology and tools for biomarker detection in cancer
Health and Society, Malmö University 2016
Doctoral thesis by Zahra El-Schich
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“To analyze morphological changes of death cells, [HoloMonitor] was used. Digital holographic microscopy is an approach for label-free non-invasive 3D imaging of cultured cells. We have analyzed cell death of adherent cancer cells using [HoloMonitor] and developed it to analyze suspension cells by combining this technique with antibody based micro­assays. [HoloMonitor] can be used for cell-death induced cell analysis of both adherent cells and suspension cells.”
Aquaporins in Infection and Inflammation
Clinical and Experimental Medicine, Linköping University 2016
Doctoral thesis by Angelika Holm
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By recording time-lapse videos directly within the incubator using HoloMonitor M4, volume changes of bacteria infected cells was studied.
Living cells and copolymer coatings exposed to green tea polyphenol (EGCg): Dynamic investigations using label-free optical biosensors
Doctoral school of molecular- and nanotechnology, University of Pannonia 2016
Doctoral thesis by Beatrix Éva Péter
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Protection of the blood-brain barrier under pathological conditions
Faculty of medicine and biological research centre, University of Szeged 2014
Doctoral thesis by Andrea Tóth
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Nanowires in Cell Biology – Exploring Interactions and Applications
Division of Solid State Physics, Lund University 2014
Doctoral thesis by Henrik Persson
Thesis Image: Cells on nanowires imaged by HoloMonitor M4
Using HoloMonitor M4 this thesis explores the interactions between cells and nanowires, to increase our under­standing of how cells are affected and how they can be manipulated by these one-dimensional, semiconductor, crystals.
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Design of Reconfigurable Hardware Architectures for Real-time Applications
Department of Electrical and Information Technology, Lund University 2008
Doctoral thesis by T. Lenart
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Holographic microscopy video showing asymmetrical cell division of JIMT-1 cells
The CELL — an imaging library 2012
Birgit Janicke
View video…
Holographic time-lapse video showing live human prostate cells (DU 145) undergoing apoptosis, induced by etoposide
The CELL — an imaging library 2012
Birgit Janicke
View video…