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Applications of quantitative time lapse holographic imaging to the development of complex pharmaceutical nano formulations

SPIE BiOS Conference Proceedings, 2018 | E Luther, L Mendes, J Pan, D Costa, C Sarasozen and V Torchilin

We rely on in vitro cellular cultures to evaluate the effects of the components of multifunctional nano-based formulations under development. We employ an incubator-adapted, label-free holographic imaging cytometer HoloMonitor M4® (Phase Holographic Imaging, Lund, Sweden) to obtain multi-day time-lapse sequences at 5- minute intervals. An automated stage allows hand-free acquisition of multiple fields of view.

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