Publications

Below you will find summaries and links to peer reviewed articles and other publications and conference papers discussing HoloMonitor applications and PHI's core technology – holographic and quantitative phase imaging (QPI) microscopy.

Peer Reviewed Articles and Book Chapters

  • Scientific Reports, 2018 | Kovács ÁF, Láng O, Turiák L, Ács A, Kőhidai L, Fekete N, Alasztics B, Mészáros T, Buzás E I and Rigó J Jr. Pállinger

    The study suggests that circulating extracellular vesicles (EVs) activate THP-1 monocytes, induce intra-monocytic TNF expression, as well as an altered chemotaxis and cell migration pattern. This is the first study to investigate in preeclampsia the effects of circulating EVs on monocyte chemotaxis, cell adhesion and migration in vitro.

    The HoloMonitor M4 was used for cell tracking of adherent cells. In the present experiments the chemokinetic effects were evaluated by measurement and calculation of migration and motility.

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  • SPIE BiOS Conference Proceedings, 2018 | E Luther, L Mendes, J Pan, D Costa, C Sarasozen and V Torchilin

    We rely on in vitro cellular cultures to evaluate the effects of the components of multifunctional nano-based formulations under development. We employ an incubator-adapted, label-free holographic imaging cytometer HoloMonitor M4® (Phase Holographic Imaging, Lund, Sweden) to obtain multi-day time-lapse sequences at 5- minute intervals. An automated stage allows hand-free acquisition of multiple fields of view.

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  • SPIE Conference Proceedings, 2018 | M Hejna, A Jorapur, Y Zhang, J S Song and R L Judson

    Working with a HoloMonitor M4 digital holographic cytometry platform, we have established a machine learning-based pipeline for high accuracy and label-free classification of adherent cells.

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  • Journal of Hazardous Materials, 2018 | E Farkasa, A Szekacsc, B Kovacsa, M Olahc, R Horvatha and I Szekacs

    In the present work, the herbicide product Roundup Classic, its formulant (POEA) and its active ingredient (glyphosate) were studied on a mammalian osteoblastic cell line (MC3T3-E1) model using the optical biosensor Epic BT. Cell viability was monitored by HoloMonitor M4 by analyzing three-dimensional morphological structures of the cells.

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  • SPIE BiOS Company Proceedings, 2018 | P Egelberg

    It is discussed how relevant biological information can be extracted from a sequence of quantitative phase images, with negligible user assistance and parameter tweaking, to simultaneously provide cell culture characteristics such as cell growth rate, viability, division rate, mitosis duration, phagocytosis rate, migration, motility and cell-cell adherence without requiring any artificial cell manipulation.

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  • Cell Death and Disease, 2018 | J Ousingsawat, P Wanitchakool, R Schreiber and K Kunzelmann

    The role of TMEM6F in pyroptosis was investigated and the data suggests that TMEM16F has a significant impact on pyroptotic cell lysis and subsequent phagocytosis by neutrofils. HoloMonito was used to analyse cell volume changes.

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  • JoVe (video journal), 2018 | J Huang, P Guo and Marsha A. Moses

    The video and publication demonstrate how HoloMonitor is used to conduct time-lapse and labeling-free characterizations of angiogenic and non-angiogenic human osteosarcoma KHOS cells. A panel of cellular parameters, including cell morphology, proliferation, and motility, were quantitatively measured and analyzed using HoloMonitor.

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  • American Journal of Physiology-Lung Cellular and Molecular Physiology, 2018 | Yao-Wen Chang et al

    Time-lapse videos and cell morphology images were recorded with a HoloMonitor M4 to assess the effects of the compound on neutrophil-platelet interaction and activation

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  • Journal of Neural Transmission , 2018 | E Lajkó, B Tuka, F Fülöp, I Krizbai, J Toldi and K Magyar

    Holomonitor M4 was used to analyse both chemokinetic responses and morphometry. The results obtained on endothelial responses
    and its amide derivatives.

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  • Neurotherapeutics, 2017 | S-X Cheng et al

    Targeted temperature management (TTM) modulation on traumatic brain injury (TBI). HoloMonitor was used to analyze cell area and cell volume after exposure of cells to hypothermia or normothermia.

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  • Nano Letters, 2017 | Y. Messica
  • Formaldehyde and Cognition, 2017 | J. Lu, R. He

    Mechanisms related to Alzheimer’s disease (AD). Formaldehyde is shown to be a strong trigger of Aβ deposition and Tau phosphorylation, so that the dysmetabolism of endogenous formaldehyde should occur in the early stage of AD as formaldehyde disturbs nervous system in different pathways and in diverse manners.

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  • Nature Communications, 2017 | J. Ju et al

    The authors report that NatD (N-α-acetyltransferase D) promotes the migratory and invasive capabilities of lung cancer cells in vitro and in vivo. On the other hand, depletion of NatD suppresses the epithelial-to-mesenchymal transition (EMT) of lung cancer cells. These findings indicate that NatD is a crucial epigenetic modulator of cell invasion during lung cancer progression.

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  • Scientific Reports, 2017 | X. Yang

    Investigating the function of zinc in stimulated cell motility and motility speed in normal cell lines. Afterwards, energy metabolism pathways and impaired energetic metabolism models were introduced to show the function of zinc in the cellular energy supply.

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  • Cell Cycle, 2017 | S. Kamlund, D. Strand, B. Janicke, K. Alm, S Oredsson

    Digital holographic microscopy was used for longitudinal cell tracking of individual cells. Various parameters were investigated and the results suggest that EMT (epithelial to mesenchymal transision)  and MET (mesenchymal to epithelial transission) can be investigated using this method.

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  • Biophysical Chemistry, 2017 | L. Petecchia, F. Viti, F. Sbrana, M. Vassalli and P. Gavazzo

    The authors wrote their own software for multi-parameter morphology analysis. Digital Holography allowed to extract a set of quantitative indicators associated with the specific biological state and, among all possible parameters, the average thickness, T, and the roughness kurtosis, RK, allowed to produce a scatter plot in which full osteoblasts, full adipocytes and undifferentiated hBM-MSC lie in well separated regions of the space. Noteworthy, this T-RK graph was successfully extended to all conditions and provided a clear and prompt visualization of the cellular commitment in terms of position in the T-RK plane.

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  • Scientific Reports, 2017 | H. Ogawa, M. Koyanagi-Aoi, K. Otani, Y. Zen, Y. Maniwa, T. Aoi

    The authors established technologies to generate lung CSC (cancer stem cell) -like cells from human lung cancer cell line A549 by introducing OCT3/4, SOX2 and KLF4, and to construct “lung cancer organoids” in vitro that mimicked human lung cancer tissues. Through the use of these technologies and the evaluation of clinical samples, interleukin-6 was identified as a novel potential therapeutic target for lung cancer stem cells.

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  • Nature Communications, 2017 | I. Yeh et al

    HoloMonitor was used to measure cell volume. Together with other methods the results identify DPN (deep penetrating nevus) as an intermediate melanocytic neoplasm, with a progression stage positioned between benign nevus and DPN-like melanoma.

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  • Experimental Cell Research, 2017 | L. Petecchia, C. Usai, M. Vassalli and P. Gavazzo

    The aim of the study was to evaluate the effectiveness of a nano-patterned titanium surface in sustaining hBM-MSC adhesion, growth and differentiation.

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  • Scientific Reports, 2017 | M. Hejna

    The authors used machine learning to develop a method for robust and kinetic label-free classification of single adherent cells info functional states.

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  • Cryobiology and Cryotechnology, 2017 | Hiroshi Ishiguro, Makoto Uemura and Kouhei Nagasaki

    This study investigated the dynamics of morphological changes, including mobility of adherent cultured cells, under low-temperature stress near physiological temperature, using time-lapse three-dimensional imaging with digital holographic microscopy (DHM).

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  • Experimental Cell Research, 2017 | L. Petecchia, C. Usai, M. Vassalli and P. Gavazzo

    The aim of the study was to evaluate the effectiveness of a nano-patterned titanium surface in sustaining hBM-MSC adhesion, growth and differentiation.

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  • Eur J Physiol, 2017 | Y. Messica

    Mechanism in cystic fibrosis; role of induced cell death by reactive oxygen species (ROS) was investigated. Cell volume measurements using HoloMonitor was used to assess cell shrinkage during cell death.

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  • Biomedicine & Pharmacotherapy, 2017 | Soundararajan Vijayarathnaa, Yeng Chenb, Jagat R. Kanwarc and Sreenivasan Sasidharana

    In this study, the apoptogenic property and the cytotoxic effect of P. longifolia leaf methanolic extract (PLME) against the human cervical carcinoma cells (HeLa) was studied using light microscope (LM), digital holographic digital microscopy (DHM), scanning electron microscope (SEM) and transmission electron microscope (TEM).

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  • Dalton Transactions 2017, 2017 | Zhao Zhang, Hua-Hua Wang, Hua-Jun Yu, Yu-Zhen Xiong, Hai-Tao Zhang, Liang-Nian Jid and Hai-Yang Liu

    After illumination and treatment with 1-Ga, A549 cells exhibited characteristics typical of apoptosis, spherical shape of the cells, chromatin condensation, and nuclear membrane irregularity. After incubation for 1 h, the authors found that the cells started to contract, the area decreased and the thickness increased.

    Read more
  • Scientific reports, 2017 | Xiao-Sa Yang, Tai-Long Yi, Sai Zhang, Zhong-Wei Xu, Ze-Qi Yu, Hong-Tao Sun, Cheng Yang, Yue Tu & Shi-Xiang Cheng

    These findings indicate that necroptosis contributes to ischemic brain injury induced by OGD and MCAO and implicate HIF-1α, RIP1, RIP3, and MLKL in necroptosis.

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  • Progress in Biochemistry and Biophysics, 2017 | Wang Jing, Zhou Jun, MO Wei-Chuan, He Ying-Ge, Wei Yan, He Rong-Qiao and Yi Fa-Ping

    Chronic formaldehyde (FA) exposure leads to impairment of human memory. Using HoloMonitor Wang Jing and coworkers illustrate how FA exposure influence the cell morphology in terms of thickness, area and volume.

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  • Experimental Cell Research, 2017 | L. Petecchia, C. Usai, M. Vassalli, and P.Gavazzo

    Evaluation of the effectiveness of a nano-patterned titanium surface in sustaining hBM-MSC adhesion, growth and differentiation by means of a panel of biophysical tools: morphometry, electrophysiology, intracellular calcium measurements and immunocytochemistry. The results substantiate the idea that this micro-textured titanium dioxide is a good surface for growth and differentiation of hBM-MSC and it exhibits a stimulating action mainly in the initial period of differentiation. HoloMonitor was used for analysis of various morphological parameters.

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  • Molecular Medicine Reports, 2017 | Xiao‑Sa Yang et al

    The present study revealed that ouabain intervention inhibited U‑87MG cell viability and cell motility in a dose‑dependent manner, suggesting that ouabain promoted the apoptosis of U87‑MG cells. HoloMonitor was used to analyse cell motility, cell count and to study the 3D cell structure.

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  • Archiv der Pharmazie, 2017 | M. Gyuris, L. Hackler, L. Nagy, R. Alföldi, E. Rédei, A. Marton, T. Vellai, N. Faragó, B. Ózsvári, A. Hetényi, G. Tóth, P. Sipos, I. Kanizsai and L. Puskás

    Structure–activity relationship (SAR) studies were performed by applying viability assays and holographic microscopic imaging to these curcumin analogues for anti-proliferative activity against A549 and H1975 lung adenocarcinoma cells. The TNFα-induced NF-κB inhibition and autophagy induction effects correlated strongly with the cytotoxic potential of the analogues. Significant inhibition of tumour growth was observed when the most potent analogue 44 was added in liposomes at one-sixth of the maximally tolerated dose in the A549 xenograft model.

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  • Biochemistry, Genetics and Molecular Biology » "New Insights into Cell Culture Technology", 2017 | M. Jedrzejczak-Silicka

    Book chapter review

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  • Chemistry — A European Journal, 2017 | Butkevich

    Cell viability in the presence of dyes in the centromeres was verified by measuring cell morphology of Drosophila S2 cells using a holographic microscope (Holomonitor M4). images and measures cells without using invasive dyes or reagents. Using HoloMonitor, the authors proved that cells stay alive and continue to divide after staining them with the improved super-resolution dyes.

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  • The journal of steroid biochemistry and molecular biology, 2017 | Olivier

    Cell morphological changes in ageing: Skin photoaging due to UV irradiation is a degenerative process that appears more and more as a growing concern. Lipids, including oxysterols, are involved in degenerative processes; as skin cells contain various lipids, the aim of our study was to evaluate first, changes in keratinocyte lipid levels induced by UV exposure and second, cellular effects of oxysterols in cell morphology and several hallmarks of keratinocyte differentiation.

    Using HoloMonitor and confocal microscopy analysis, the authors highlighted cell thickening and cytoskeletal damage after incubation of keratino­cytes with 25-hydroxycholesterol.

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  • Cytometry Part A 2017, 2017 | Luther E et al

    A review of HoloMonitor applications: tracking of Giant HeLa cells, which may be undergoing neosis tracking the effects of cell cycle related toxic agents on cell lines; using MicroRNAs to reverse the polarization state in macrophages to induce tumor cell killing development of liposomal nanoformulations to overcome multi-drug resistance in ovarian cancer cells and development of dual sensitive micelles to specifically target matrix metalloproteinase 2

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  • Cytometry Part A 2017, 2017 | Janicke

    The authors have developed a robust and label-free kinetic cell proliferation assay with high temporal resolution for adherent cells using HoloMonitor M4. Only two image processing settings were adjusted between cell lines, making the assay practical, user friendly, and free of user bias. In the recorded time-lapse image sequences, individual cells were automatically identified to provide detailed growth curves and growth rate data of cell number, confluence, and average cell volume. The results demonstrate how these parameters facilitate a deeper understanding of cell processes than what is achievable with current single-parameter and end-point methods.

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  • PLOS ONE, 2017 | Hsuen-Wen Chang, Zih-Miao Lin, Min-Ju Wu, Li-Yu Wang, Yen-Hung Chow, Shih Sheng Jiang, Hui-Ju Ch’ang and Vincent HS Chang

    The authors have developed a mouse model which is very similar to human, and which can be used for more relevant drug evaluations. Focus are is tumor metatasis and especially the phenomenon of the epithelial-mesenchymal transition (EMT). HoloMonitor was used to investigate the capability of cell movement of tumor cells at different stages.

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  • Scientific Reports, 2017 | Denian Wang, Chun Tan, Fei Xiao, Lan Zou, Lijun Wang, Yong’gang Wei, Hanshuo Yang, and Wei Zhang

    Even if the blood supply is cut off in tumors, cells will survive, and even migrate, and maybe cause increased metastasis.

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  • Cytometry Part A, 2017 | Anette Gjörloff-Wingren

    Prof. Gjörloff-Wingren has used holographic microscopy in cancer related research for over a decade. Gjörloff-Wingren discuss the emerging field of quantitative phase imaging and the novel label-free capabilities that promise to improve and succeed invasive laborious photochemical procedures

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  • Biomedicine & Pharmacotherapy, 2017 | Nowroji Kavitha, Yeng Chen, Jagat R. Kanwar and Sreenivasan Sasidharan

    The purpose of the study was to determine the cytotoxic effect of P. macrocarpa fruit ethyl acetate fraction (PMEAF) by using various conventional and modern micro­scopy techniques. The results indicate that PMEAF is a potential anticancer agent and a candidate for developing more potent anticancer drugs.

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  • Food and Chemical Toxicology, 2017 | Aysun Özdemir, Metin Yildiz, Fatma Sezer Senol, Yaprak Dilber Şimay, Burçin Ibişoglu, Alper Gokbulut, Ilkay Erdogan Orhan and Mustafa Ark

    Aim was to study the impact of plant extracts from Cyclotrichium niveumon on normal-like and cancer cell lines. The authors concluded that C. niveum has cytotoxic properties in the cancer cell lines tested.

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  • Cytometry Part A, 2017 | Peng Guo, Jing Huang and Marsha A. Moses

    Based on HoloMonitor M4, the authors developed a novel in vitro method to characterize the angiogenic and non-angiogenic phenotypes of human osteosarcoma cells. Compared with traditional in vitro assays, the approach provides a non-invasive and low-cost method to assess a panel of cell morphology and cell behaviors. The authors were able to easily and accurately differentiate between the angiogenic and non-angiogenic phenotypes of KHOS cells by comparing five cellular parameters: cell area, cell thickness, cell migration, cell motility and cell motility speed.

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  • Dalton Transactions, 2017 | Zhao Zhang, Hua-Hua Wang, Hua-Jun Yu, Yu-Zhen Xiong, Hai-Tao Zhang, Liang-Nian Jid and Hai-Yang Liu

    After illumination and treatment with 1-Ga, A549 cells exhibited characteristics typical of apoptosis, spherical shape of the cells, chromatin condensation, and nuclear membrane irregularity. After incubation for 1 h, the authors found that the cells started to contract, the area decreased and the thickness increased.

    Read more
  • Holographic Materials and Optical Systems 2017, 2017 | El-Schich

    Open access book chapter discussing the potential of holographic microscopy in clinical applications

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  • Nature Communications, 2017 | Yuan Gao, Zhaowei Wang, Qiang Hao, Weina Li, Yujin Xu, Juliang Zhang, Wangqian Zhang, Shuning Wang, Shuo Liu, Meng Li, Xiaochang Xue, Wei Zhang, Cun Zhang and Yingqi Zhang

    The authors tracked and analyzed the migration of different MCF-7 cell populations in a 3D matrix. In addition to measuring the cell migration speed with HoloMonitor, the following five phenotypes were observed: ‘stable adhesion’, ‘unstable lamellipodia’, ‘bleb’, ‘stable bleb’ and ‘unstable pseudopod’.

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  • Journal of Southern Medical University, 2017 | Yu JH, Liu XL, Liu YJ, He XE, Hui Y, Zhang B, Zhu L and Zhao W

    Digital holographic microscopy allows monitoring of the complex morphological changes of cells during DENV infection. The 4 DENV strains with different serotypes causes characteristic cell damages during infection

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  • Journal of Medical Imaging, 2017 | Anna Leida Mölder, Johan Persson, Zahra El-Schich, Silvester Czanner, Anette Gjörloff-Wingren

    The morphological changes observed occur before and at lower concentrations than a reduction in cell metabolic activity or viability. Three classifiers are compared and we report a best case sensitivity of 88% and specificity of 94% for classification of cells as treated/untreated.

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  • The faseb journal , 2016 | J. Sun, M. Xu, H. Ortsäter, E. Lundeberg, L. Juntti-Berggren, Y. Q. Chen, J. Z. Haeggström, G. H. Gudmundsson, J. Diana and B. Agerberth

    HoloMonitor M4 was used to assess the cell density during cell proliferation.

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  • International journal of radiation biology , 2016 | Kawase

    Cell morphological parameters (e.g. cell volume) monitored by HoloMonitor could be useful and more stable than the DNA damage markers currently used.

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  • Molecular cancer therapeutics , 2016 | Sriraman

    To follow the development of mitotic arrest followed by apoptosis in real time (only visual evaluation?)

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  • Cellular and molecular life sciences , 2016 | Ousingsawat

    It is well known that cells swell during necroptosis. However, proper cell volume measurements are still missing. Using Holo­Monitor M4 the authors non-invasively measured the cell volume changes associated with necroptosis. The results were confirmed by flow cytometry measurements.

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  • European journal of physiology, 2016 | Benedetto

    Analysis of hypo-induced changes in cell height rather than cell volume, as an increase in cell height is not compromised by basal adhesions or cell/cell contacts. Cell height measured by holographic microscopy in HEK293 cells, indicated hypotonic cell swelling (Hypo) within seconds and completed regulatory volume decrease (RVD) within less than 2 min

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  • Small , 2016 | Guseppina Salzano, Daniel F. Costa, Can Sarisozen, Ed Luther, George Mattheolabakis, Pooja P. Dhargalkar, and Vladimir Torchilin

    Methods for producing 4D plot were developed. Images from the time course were exported to ImageJ and treated as an image stack, creating a volume rendering of the cellular motion over time of analysis. The brightness threshold was adjusted to view all cells within the imaging area or only the thicker cells, a feature that allows identification of mitosis, apoptosis, and transition between mesenchymal and amoeboid cellular morphologies

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  • European biophysics journal, 2016 | Beatrix Peter, Szilvia Bosze and Robert Horvath

    HoloMonitor-based methods and other methods to assess cell adhesion, motility, migration, stiffness, apoptosis and proliferation exposed to green tea polyphenol epigallocatechin-3-gallate are reviewed.

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  • Advanced sciences and technologies for security applications, 2016 | Inna Székács, Robert Horvath and András Székács
  • Cellular and molecular life sciences , 2016 | Kunzelmann

    Despite the rather different morphologic appearances of apoptosis and necroptosis, both mechanisms of cell death require the transport of ions, organic osmolytes, and water. The contribution of these transport proteins and underlying mechanisms are summarized in this review.

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  • Scientific reports , 2016 | W-C. Mo, Z-J. Zhang, D-L. Wang, Y. Liu, P. Bartlett and R-Q. He

    Using holomonitor M4, the authors show that the elimination of Earth’s magnetic field affects cell behavior. Potential influence on cancer treatments suggested.

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  • Frontiers in cellular and infection microbiology , 2016 | A. Holm, K-E. Magnusson and E. Vikström

    Analysis of optical cell volume changes; time-lapse videos were recorded using the holomonitor M4 Image acquisition was set at an interval of 30 s for 1 h.

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  • Plos one , 2016 | Hackler

    Visualization of cytotoxic effects using holomonitor. These experiments record morphological parameters of treated cells such as area, thickness and volume. First time where the effects of drug induced cell death on cellular morphology of these glioma cells were detected by using 3D holographic images.

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  • J. Biomed. Opt , 2015 | Beatrix Peter, Judit Nador, Krisztina Juhasz, Agnes Dobos, Laszlo Korosi, Inna Székács, Daniel Patko and Robert Horvath

    Holomonitor M4 was used to observe cancer cell motility, motility speed, and migration in the presence of the green tea polyphenol, epigallocatechin gallate, as well as to monitor the adhesion of preosteoblast cells on nanostructured titanate coatings, relevant for biomedical applications.

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  • Molecular pharmaceutics , 2015 | S. K. Sriraman, J. Pan, C. Sarisozen, E. Luther and V. Torchilin

    Using holomonitor M4, it was found that FA-(C6+Dox)-LP treated hela cells underwent rapid progression to apoptosis (cell suicide) after 21 hours, as evidenced by a drastic drop in cell area after loss of cell membrane integrity.

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  • Scientific reports , 2015 | H. Persson, Z. Li, J. O. Tegenfeldt, S. Oredsson and C. N. Prinz

    Under­standing of the inter­actions between cells and nanowires. The results provide guidelines to minimize cellular stress on nanowire arrays. Additionally, the findings show that cell behavior can be controlled by adjusting nanowire density, which may have applications in drug development.

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  • Cancer cell international , 2015 | E. Lajkó, P. Bányai, Z. Zámbó, L. Kursinszki, É. Szőke and L. Kőhidai

    Our results highlight the effective and selective inhibitory activity of purpurin towards melanoma cells and its possible use as a targeted anticancer agent. The anthraquinones of the cytotoxic extract are suggested to apply in drug delivery systems as an anticancer drug

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  • International journal of pharmaceutics, 2015 | N. Zeng, N. Mignet, G. Dumortier, E. Olivier, J. Seguin, M. Maury, D. Scherman, P. Rat and V. Boudy

    The assessment of cell viability (MTT, Alamar Blue), membrane integrity (Neutral Red), and apoptosis assay (Hoechst 33342), were performed and associated to holomonitor M3 analysis.

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  • The faseb journal , 2015 | Shravan Kumar Sriraman, Yilin Zhang, Ernst Lengyel and Vladimir Torchilin

    Using time-course holographic imaging studies, this cell cycle arrest followed by progression to apoptosis was clearly visualized and confirmed.

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  • Journal of Clinical & Cellular Immunology , 2015 | Oka A, Talekar M, Ouyang Q, Luther E and Amiji M

    Holographic assessment using Holomonitor M4 of the co-cultured cells in real-time showed differences in motility and morphology of macrophages with mir-155 ME treated cells showing greater cellular interaction between the two phenotypes.

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  • Future science oa, 2015 | Zahra El-Schich, Emmy Nilsson, Anna S Gerdtsson, Christer Wingren and Anette Gjörloff Wingren

    The article provides proof of concept for using holographic microscopy combined with antibody-based microarray technology for detecting morphological changes in captured cells.

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  • Journal of structural biology, 2015 | Zahra El-Schich, Anna Mölder, Helena Tassidis, Pirkko Härkönen, Maria Falck Miniotis and Anette Gjörloff Wingren

    Using HoloMonitor M3, the authors show that holographic microscopy allows label-free and completely non-invasive morphological measurements of cell growth, viability and death.

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  • International Journal of Agricultural, Biosystems Science and Engineering, 2014 | I. Székács et al.

    The article describes a study of the health effects of the well-known, widely used herbicide Roundup. In the study, the HoloMonitor M4 was used to assess toxic effects to animal cells after exposure to Roundup.

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  • PLOS ONE, 2014 | Maria Falck Miniotis, Anthonny Mukwaya and Anette Gjörloff Wingren

    We show that average cell phase volume results from DHM readings are comparable to the flow cytometry findings. DHM thus provides a non-disruptive alternative to flow cytometry. The technique has the potential to develop into a fast and cost-efficient method for pre-clinical monitoring of cancer treatment efficacy.

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  • Lipids in health and disease, 2014 | Otilia Antal, László Hackler, Junhui Shen, Imola Mán, Katalin Hideghéty, Klára Kitajka and László G Puskás

    Holomonitor M3 was used to assess the increased death of glioma (brain tumor) cells when combining irradiation with various unsaturated fatty acid treatments

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  • PNAS, 2014 | J. Semenas et al

    The Holomonitor M3 imaging system was applied to capture living PC-3 cells in culture during treatment

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  • PLOS ONE, 2014 | Andrea E. Tóth et al

    Using holographic microscopy for label-free analysis of living cells, visualized for the first time the kinetics of morphological changes caused by methylglyoxal. The morphology data confirmed that edavarone completely prevented methylglyoxal-induced changes in morphology and detachment of cells.

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  • Urologic Oncology, 2014 | M Sarwara, S Sandberg, P-A Abrahamsson, and J L Persson

    Aim of the study was to explore a suggested signalling pathway in the progression of prostate cancer. HoloMonitor M3 was used for imaging of cells and for analysis of morphological parameters.

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  • Small, 2013 | H. Persson, C. Købler, K. Mølhave, L. Samuelson, J. O. Tegenfeldt, S. Oredsson and C. N. Prinz

    Fibroblast behaviour on vertical nanowire arrays was investigated using the Holo­Monitor M3. It was shown that cell motility and proliferation rate are reduced on nanowires. Fibroblasts cultured on long nanowires exhibit failed cell division, DNA damage, increased ROS content and respiration.

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  • Holography — Basic Principles and Contemporary Applications , 2013 | K. Alm, Z. El-Schich, M. Falck Miniotis, A. Gjörloff Wingren, B. Janicke and S. Oredsson
  • J. Of Biological Chemistry, 2013 | J. C. S. Ho, P. Storm, A. Rydström, B. Bowen, F. Alsin, L. Sullivan, I. Ambite, K. H. Mok, T. Northen and C. Svanborg

    The authors used the holomonitor M3 to observe morphological differences on cells over time, treated with either Hamlet or various lipids associated with HAMLET.

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  • PLOS One, 2013 | P. Storm, T. K. Klausen, M. Trulsson, J. C. S Ho, M. Dosnon, T. Westergren, Y. Chao, A. Rydström, H. Yang, S. F. Pedersen and C. Svanborg

    Changes in cell morphology was studied using HoloMonitor technology

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  • Gut, 2013 | M. Puthia, P. Storm, A. Nadeem, S. Hsiung, C. Svanborg

    Using the HoloMonitor M2, the authors show that cell thickness and adherent surface area change drama­tically after HAMLET treatment. HAMLET is a human α-lactalbumin made lethal to tumor cells.

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  • PLOS ONE, 2012 | J. C. S. Ho, A. Rydström, M. S. S. Manimekalai, C. Svanborg and G. Grüber

    Peptides covering the alpha1 and alpha2 domains of the protein triggered rapid ion fluxes in the presence of sodium oleate and were internalized by tumor cells, causing rapid and sustained changes in cell morphology. The alpha peptide-oleate bound forms also triggered tumor cell death with comparable efficiency as HAMLET. In addition, shorter peptides corresponding to those domains are biologically active.

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  • Future Medicine, 2012 | J. C. S. Ho, A. Rydström, M. Trulsson, J. Bålfors, P. Storm, M. Puthia, A. Nadeem and C. Svanborg
  • Cancer Research, 2012 | M. A. Thelin, K. J. Svensson, X. Shi et al

    Chondroitin and dermatan sulfate proteoglycans, which occur both in the matrix and at the cell surface, play important roles in thetumorgenic processes. The unique feature that distinguishes DS from CS is the presence of iduronic acid (IdoA) in DS. IdoA in DS influences tumorigenesis by affecting cancer cell behavior

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  • PLOS ONE, 2011 | M. Trulsson, H. Yu, L. Gisselsson, Y. Chao, A. Urbano, S. Aits, A-K. Mossberg, C. Svanborg

    Interaction between HAMLET and a-actinins promotes tumor cell detachment.

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  • Holography-Research and technologies, 2011 | K. Alm, H. Cirenajwis, L. Gisselsson, A. Gjörloff Wingren, B. Janicke, A. Mölder, S. Oredsson, J. Persson
  • PNAS, 2010 | David Gisselsson Nord et al.

    Gisselsson and colleagues studied cell division in short-term cultures established from fibroblasts and primary tumors. The researchers showed that abnormal cell divisions can cause an abnormal number of chromosomes in cancer cells.

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  • Anticancer Drugs, 2010 | Cirenajwis et al.

    Cirenajwis and colleagues used the label-free live cell imaging capabilities of the HoloMonitor to study the movement of JIMT-1 breast cancer cells. They showed that treatment with the ornithine analogue difluoromethylor-nithine (DFMO) increased cell motility, while treatment with the polyamine analogue PG-11047 decreased cell motility.

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  • Microscopy: Science, Technology, Applications and Education, 2010 | J. Persson, A. Mölder, S-G. Pettersson, K. Alm

    Using digital holography in combination with our program Celltrack, we can follow a population of cells, and we can study single cells within that population. The results also show that we can correlate cell movement with the level of confluence in the cultures, cell area, cell optical thickness and cell volume. In all, digital holography proved suitable for a cell movement assay.

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  • Vaccine, 2010 | S. Broos, K. Lundberg, T. Akagi, K. Kadowaki, M. Akashi, L. Greiff, C. A. Borrebaeck, M. Lindstedt
  • Microscopy: Science, Technology, Applications and Education, 2010 | Z. El-Schish, A. Mölder, M. Sebesta, L. Gisselsson, K. Alm, A. Gjörloff Wingren
  • Journal of Microscopy, 2008 | Anna Mölder et al

    Using a unique non-invasive labelfree cell counting method, results comparable to conventional cell counting using a haemocytometer were produced. The major advantage using HolomonitorTM M2 is the opportunity to easily access information about cell number, size, optical thickness and confluence in an automatic, non-invasive manner.

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  • Journal of VLSI Signal Processing Systems, 2008 | T. Lenart, M. Gustafsson, V. Öwall
  • IEEE, 2008 | Thomas Lenart, Henrik Svensson and Viktor Öwall
  • Optics Letters, 2005 | M. Sebesta, M. Gustafsson
  • Optics and Lasers in Engineering, 2004 | M. Gustafsson, M. Sebesta, B. Bengtsson, S.G. Pettersson, P. Egelberg, T. Lenart
  • Applied Optics, 2004 | M. Gustafsson, M. Sebesta

Conference Papers and Posters

  • AACR-NCI-EORTC Inter. Conf. on Molecular Targets and Cancer Therapeutics 2015, 2018 | Ed Luther, Giuseppina Salzano, Shravan Kumar Sriraman, Daniel Costa and Vladimir P. Torchilin
  • AACR Annual Meeting, 2017 | Ed Luther, Livia Mendes, Jiayi Pan, Daniel Costa, Elena Holden and Vladimir Torchilin
    4-dimensional plots of HoloMonitor time-lapse data were developed to assist in the visualization of the effects of pharmaceutical compounds under development.
  • AACR Annual Meeting , 2017 | Hanna Wallin, Kersti Alm and Magnus Abrahamson
    Analysis using HoloMonitor revealed qualitative differences in the normal behavior of the A375 cells under study as a result of cystatin C addition. The motility of the cells was significantly affected. The mitosis phase was considerably prolonged in cells grown in cystatin C containing medium, indicating that the proliferation-inhibiting effect of the protease inhibitor may be through its direct action on proteolytic processes in the mitosis phase.
  • AACR Annual Meeting , 2016 | J. K. Das and D. Roy
    Chemokine receptor CXCR4 is involved in the maintenance of stemness property of stem cells, aging and metastasis of cancer. NRF1 is one of the major transcription factors that controls transcription of CXCR4. In this study, the authors have investigated whether transcription regulation of CXCR4 by NRF1 controls estrogen-induced malignant transformation of breast epithelial cells to breast cancer stem cells.
  • 3rd Conference on Impedance-Based Cellular Assays, 2016 | Ed Luther, Livia Mendes, Jiayi Pan, Daniel Costa, Elena Holden and Vladimir Torchilin
    “Our objective is to develop multi-functional nanotechnology-based antitumor drug delivery systems for improving efficacy of treatments and reducing undesirable side effects. The essential part of this process is the development of un-biased quantitative analytical techniques. We are reporting a successful validation of a very high content, medium throughput system in multi-well plates, employing the newly developed holographic imaging cytometer HoloMonitor® M4 for label-free time-lapse cellular analysis.”

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  • 3rd Conference on Impedance-Based Cellular Assays, 2016 | E. Lajkó, S. Spring, O. Láng, S. Ingebrandt, G. Mező and L. Kőhidai

    HoloMonitor M4 was used for the morphometry and migration studies.

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  • AACR Annual Meeting, 2015 | Ed Luther, Meghna Talekar, Adwait Oka, Qijun Ouyang, Mansoor Amiji.
  • AACR Annual Meeting, 2015 | Ed Luther, Jeffrey Agar and Mansoor Amiji

Doctoral Theses

  • Tina Heumann, 2017 | Faculty of Biosciences, Heidelberg University
    Morphological changes were evaluated by comparing time-plots of cell volume and thickness, created by HoloMonitor M4.
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  • Judit Nádor, 2016 | Institute of Technical Physics and Material Science, Center for Energy Research, Hungarian Academy of Sciences

    The HoloMonitor M4 was used to monitor living cells (human embryonic kidney cells and preosteoblasts) on nanostructured coatings in a novel cuvette configuration.

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  • Angelika Holm, 2016 | Clinical and Experimental Medicine, Linköping University
    By recording time-lapse videos directly within the incubator using HoloMonitor M4, volume changes of bacteria infected cells was studied.
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  • Zahra El-Schich, 2016 | Health and Society, Malmö University
    To analyze morphological changes of death cells, HoloMonitor was used. Digital holographic microscopy is an approach for label-free non-invasive 3D imaging of cultured cells. We have analyzed cell death of adherent cancer cells using HoloMonitor and developed it to analyze suspension cells by combining this technique with antibody based micro­assays. HoloMonitor can be used for cell-death induced cell analysis of both adherent cells and suspension cells.
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  • Otilia Antal, 2016 | Department of Pharmaceutical Technology, University of Szeged
    HoloMonitor M3 was used to assess the increased death of glioma (brain tumor) cells when combining irradiation with various unsaturated fatty acid treatments.
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  • Alexandra Bocsik, 2016 | Department of Pharmaceutical Technology, University of Szeged
    Morphological changes in peptide treated Caco-2 epithelial cells were analyzed using HoloMonitor technology.
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  • Andrea Tóth, 2014 | University of Szeged, Faculty of Medicine and Biological Research Centre Hungarian Academy of Sciences, Szeged

    Endothelial cells were cultured on collagen coated culture dishes with borosilicate glass bottom. HoloMonitor was used to analyze cell morphological changes before and during treatments.

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  • Henrik Persson, 2014 | Division of Solid State Physics, Lund University
    Using HoloMonitor M4 this thesis explores the interactions between cells and nanowires, to increase our under­standing of how cells are affected and how they can be manipulated by these one-dimensional, semiconductor, crystals.
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  • T. Lenart, 2008 | Department of Electrical and Information Technology, Lund University
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