The HoloMonitor® time-lapse cytometer monitors changes in cell morphology and cell counts non-invasively over time. The analysis includes data for both individual cells and entire populations at the same time, allowing for non-invasive, fast, easy and reliable toxicity studies.

Cell counting, confluence and morphology are useful parameters for toxicity analysis. The HoloMonitor time-lapse cytometer determine cell proliferation, both by cell counting and by confluence, but also allows for quantitative assessments of cell morphology. Healthy cells are irregular and rather thin, dying cells are small, less irregular and thick, while dead cells are round and thin. These features are the basics for determination of drug effects on cell populations.

In this application note we demonstrate the usefulness of HoloMonitor for toxicity studies, by showing the effect of the cancer drug etoposide on mouse fibroblast cells.