Growth Inhibition as a Response to Cytotoxicity
Cytotoxicity assays are widely used in fundamental research and in drug discovery to screen libraries for toxic compounds. Cytotoxic effects may include cell death, by inducing necrosis or apoptosis, or growth inhibition, stopping cells from actively growing and dividing. As a result, cell proliferation is decreases. Cell counting, confluence and morphology are useful parameters for toxicity analysis.
Untreated L929 cells as compared to cells treated with etoposide.
Mean cell count and confluence for L929 cells treated with various concentrations of etoposide vs control (blue line). Data from Janicke et al. Label-free high temporal resolution assessment of cell proliferation using digital holographic microscopy. Cytometry part A 2017.