Applications

Live Cell Analysis – Label Free and Quantitative

Explore our range of applications for label-free, long-term quantitative analysis of living cell dynamics, circumventing the drawbacks of traditional methods requiring toxic stains.

Cell Growth

Assess your cell culture integrity and kinetic cell proliferation:

  • Quick and quantitative cell culture QC (quality control)
  • Kinetic cell count and cell confluence
  • Cellular growth rate and morphological changes
  • Cellular population statistics

Data for untreated L929 mouse fibroblasts.

Cell Movements

Monitor cell movement patterns and distinguish between random cell motility and directed cell migration.

  • Robust segmentation and tracking of cells of interest
  • Cell motility speed and cell migration velocity measurements
  • Detailed understanding of wound healing processes

Holographic images and XY movement plots of murine macrophages M1 phenotype (left) and M2 phenotype (right).

Cell Cycles

Analyze cells through rounds of replication:

  • No perturbation to the natural state and function of the cell with label-free analysis
  • Robust measurement of mitosis and cytokinesis based on reliable automated segmentation
  • Cytometric data with effortless relationship between images and quantitative data

Changes in optical volume over time based on tracking of one cell of interest.

Drug Responses

Analyse cytotoxic effects and understand the cell death process:

  • Label-free and kinetic assessment of  changes in cell morphology and cell proliferation.
  • Determine cell proliferation, both by cell counting and by confluence, for various cell treatments simultaneously
  • Understand drug effects through quantitative assessments of cell morphology

Etoposide-treated DU145 cell line: changes in optical thickness and volume during the death process in one individual cell.

Drug Responses Assays

Other Cellular Events

HoloMonitor provides a combination of quantitative and visual data collected over time. The data can be accompanied with supporting videos which visualize how cancer cells shrivel up and die after being treated with a cancer drug, for example. Some features essential for capturing rare or transient cellular events like phagocytosis, cell engulfment, and cell activation and differentiation, are:

  • Speed of image acquisition of up to 1 image per second.
  • Morphological and quantitative tracking of cells of interest over multiple days.
  • Cell-by-cell population data analysis.

Examples how HoloMoniotor has been used to visualize previously unseen events are provided here. Explore our list of publications describing how Holomonitor can be used in various research settings. 

L929 mouse fibroblasts: a rare giant budding multinuclear cell.

Example of Other Events 

  • Cell Engulfment
  • Phagocytosis
  • Cell Activation
  • Cell Differentiation