Northeastern University – Holographic Imaging Cytometry Program of Excellence
The joint program of excellence between Northeastern University and PHI began in late 2014. Since then HoloMonitor technology has rapidly established itself as a technology of choice for label-free kinetic cellular analysis. The program covers several activities including:
- Establishing a network of collaborators utilizing Holographic Imaging Cytometry.
- Developing novel applications supporting research interests of the Northeastern faculty and students.
- Presenting results at scientific meetings.
- Organizing an annual technology symposium and hands-on laboratory courses.
Peer Reviewed Articles and Book Chapters
SPIE BiOS Conference Proceedings, 2018 |
We rely on in vitro cellular cultures to evaluate the effects of the components of multifunctional nano-based formulations under development. We employ an incubator-adapted, label-free holographic imaging cytometer HoloMonitor M4® (Phase Holographic Imaging, Lund, Sweden) to obtain multi-day time-lapse sequences at 5- minute intervals. An automated stage allows hand-free acquisition of multiple fields of view.
Cytometry Part A 2017, 2017 |
A review of HoloMonitor applications: tracking of Giant HeLa cells, which may be undergoing neosis tracking the effects of cell cycle related toxic agents on cell lines; using MicroRNAs to reverse the polarization state in macrophages to induce tumor cell killing development of liposomal nanoformulations to overcome multi-drug resistance in ovarian cancer cells and development of dual sensitive micelles to specifically target matrix metalloproteinase 2
Molecular cancer therapeutics , 2016 |
To follow the development of mitotic arrest followed by apoptosis in real time (only visual evaluation?)
Small , 2016 |
Methods for producing 4D plot were developed. Images from the time course were exported to ImageJ and treated as an image stack, creating a volume rendering of the cellular motion over time of analysis. The brightness threshold was adjusted to view all cells within the imaging area or only the thicker cells, a feature that allows identification of mitosis, apoptosis, and transition between mesenchymal and amoeboid cellular morphologies
Molecular pharmaceutics , 2015 |
Using holomonitor M4, it was found that FA-(C6+Dox)-LP treated hela cells underwent rapid progression to apoptosis (cell suicide) after 21 hours, as evidenced by a drastic drop in cell area after loss of cell membrane integrity.
Journal of Clinical & Cellular Immunology , 2015 |
Holographic assessment using Holomonitor M4 of the co-cultured cells in real-time showed differences in motility and morphology of macrophages with mir-155 ME treated cells showing greater cellular interaction between the two phenotypes.
Conference Papers and Posters
- AACR-NCI-EORTC Inter. Conf. on Molecular Targets and Cancer Therapeutics 2015, 2018 |
AACR Annual Meeting, 2017 |
4-dimensional plots of HoloMonitor time-lapse data were developed to assist in the visualization of the effects of pharmaceutical compounds under development.
3rd Conference on Impedance-Based Cellular Assays, 2016 |
“Our objective is to develop multi-functional nanotechnology-based antitumor drug delivery systems for improving efficacy of treatments and reducing undesirable side effects. The essential part of this process is the development of un-biased quantitative analytical techniques. We are reporting a successful validation of a very high content, medium throughput system in multi-well plates, employing the newly developed holographic imaging cytometer HoloMonitor® M4 for label-free time-lapse cellular analysis.”
- AACR Annual Meeting, 2015 |
- AACR Annual Meeting, 2015 |
Department of Pharmaceutical Sciences
School of Pharmacy
Bouvé College of Health Sciences
140 The Fenway, Room 228/229
60 Huntington Avenue
Boston, Massachusetts 02115