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Northeastern University – Holographic Imaging Cytometry Program of Excellence

The joint program of excellence between Northeastern University and PHI began in late 2014. Since then HoloMonitor technology has rapidly established itself as a technology of choice for label-free kinetic cellular analysis. The program covers several activities including:

  • Establishing a network of collaborators utilizing Holographic Imaging Cytometry.
  • Developing novel applications supporting research interests of the Northeastern faculty and students.
  • Presenting results at scientific meetings.
  • Organizing an annual technology symposium and hands-on laboratory courses.

Achievements

In May 2017, a summary of the scientific achievements was published in a special issue of Cytometry part A, dedicated to holographic microscopy and quantitative phase imaging.

Read the summary here and view the associated CYTO 2017 presentation here.

 

 

Location

Department of Pharmaceutical Sciences 
School of Pharmacy 
Bouvé College of Health Sciences 
Northeastern University 
140 The Fenway, Room 228/229 
60 Huntington Avenue 
Boston, Massachusetts 02115

Poster session at AACR Annual Meeting 2017. View poster presented by Ed Luther, Supervisor Core Imaging and Cytometry Facility at Northeastern University, here.

“The expansion of our existing collaboration with PHI is the result of productive utilization of HoloMonitor technology for non-invasive real-time cell cycle analysis in living cells. We are now developing state-of-the art applications multiplexing cell cycle and cell motility.”

Ed Luther, Northeastern University

Peer Reviewed Articles and Book Chapters

  • SPIE BiOS Conference Proceedings, 2018 | E Luther, L Mendes, J Pan, D Costa, C Sarasozen and V Torchilin

    We rely on in vitro cellular cultures to evaluate the effects of the components of multifunctional nano-based formulations under development. We employ an incubator-adapted, label-free holographic imaging cytometer HoloMonitor M4® (Phase Holographic Imaging, Lund, Sweden) to obtain multi-day time-lapse sequences at 5- minute intervals. An automated stage allows hand-free acquisition of multiple fields of view.

    Read more
  • Cytometry Part A 2017, 2017 | Luther E et al

    A review of HoloMonitor applications: tracking of Giant HeLa cells, which may be undergoing neosis tracking the effects of cell cycle related toxic agents on cell lines; using MicroRNAs to reverse the polarization state in macrophages to induce tumor cell killing development of liposomal nanoformulations to overcome multi-drug resistance in ovarian cancer cells and development of dual sensitive micelles to specifically target matrix metalloproteinase 2

    Read more
  • Molecular cancer therapeutics , 2016 | Sriraman

    To follow the development of mitotic arrest followed by apoptosis in real time (only visual evaluation?)

    Read more
  • Small , 2016 | Guseppina Salzano, Daniel F. Costa, Can Sarisozen, Ed Luther, George Mattheolabakis, Pooja P. Dhargalkar, and Vladimir Torchilin

    Methods for producing 4D plot were developed. Images from the time course were exported to ImageJ and treated as an image stack, creating a volume rendering of the cellular motion over time of analysis. The brightness threshold was adjusted to view all cells within the imaging area or only the thicker cells, a feature that allows identification of mitosis, apoptosis, and transition between mesenchymal and amoeboid cellular morphologies

    Read more
  • Molecular pharmaceutics , 2015 | S. K. Sriraman, J. Pan, C. Sarisozen, E. Luther and V. Torchilin

    Using holomonitor M4, it was found that FA-(C6+Dox)-LP treated hela cells underwent rapid progression to apoptosis (cell suicide) after 21 hours, as evidenced by a drastic drop in cell area after loss of cell membrane integrity.

    Read more
  • Journal of Clinical & Cellular Immunology , 2015 | Oka A, Talekar M, Ouyang Q, Luther E and Amiji M

    Holographic assessment using Holomonitor M4 of the co-cultured cells in real-time showed differences in motility and morphology of macrophages with mir-155 ME treated cells showing greater cellular interaction between the two phenotypes.

    Read more

Conference Papers and Posters

  • AACR-NCI-EORTC Inter. Conf. on Molecular Targets and Cancer Therapeutics 2015, 2018 | Ed Luther, Giuseppina Salzano, Shravan Kumar Sriraman, Daniel Costa and Vladimir P. Torchilin
  • AACR Annual Meeting, 2017 | Ed Luther, Livia Mendes, Jiayi Pan, Daniel Costa, Elena Holden and Vladimir Torchilin
    4-dimensional plots of HoloMonitor time-lapse data were developed to assist in the visualization of the effects of pharmaceutical compounds under development.
  • 3rd Conference on Impedance-Based Cellular Assays, 2016 | Ed Luther, Livia Mendes, Jiayi Pan, Daniel Costa, Elena Holden and Vladimir Torchilin
    “Our objective is to develop multi-functional nanotechnology-based antitumor drug delivery systems for improving efficacy of treatments and reducing undesirable side effects. The essential part of this process is the development of un-biased quantitative analytical techniques. We are reporting a successful validation of a very high content, medium throughput system in multi-well plates, employing the newly developed holographic imaging cytometer HoloMonitor® M4 for label-free time-lapse cellular analysis.”

    View poster

  • AACR Annual Meeting, 2015 | Ed Luther, Meghna Talekar, Adwait Oka, Qijun Ouyang, Mansoor Amiji.
  • AACR Annual Meeting, 2015 | Ed Luther, Jeffrey Agar and Mansoor Amiji

Featured Applications

Chemoresistance, by Northeastern University

Macrophage Polarization, by Northeastern University

Macrophage Response to Polarization, by Northeastern University

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