PHI and Malmö University have a long-standing collaboration, dating back to 2008. The collaboration has resulted in several peer reviewed publications and a doctoral thesis. In late 2016, the European Commission granted 2.1 million euro to GlycoImaging – a joint cancer research project to develop improved methods for clinically diagnosing cancer.
Current methods for diagnosing cancer primarily focus on the proteins associated with cancer. However, there is increasing evidence that carbohydrates play an important role in the development and progression of malignant cancer. Current methods use and rely on antibodies created by living organisms. These natural antibodies, however, are not sufficiently specific to accurately detect and image carbohydrates.
The GlycoImaging project is coordinated by Malmö University and commercialized by PHI. Additional partners are Bundesanstalt für Materialforschung und Prüfung (Germany’s federal technology research institute), Umeå, Copenhagen and Turku University.
Popular lecture on cancer research by Prof. Anette Gjörloff Wingren 2016 (in Swedish).
The GlycoImaging video (in Swedish).
A short presentation of GlycoImaging by Prof. Anette Gjörloff Wingren (in Swedish).
Peer Reviewed Articles and Book Chapters
Cytometry Part A, 2017 |
Prof. Gjörloff-Wingren has used holographic microscopy in cancer related research for over a decade. Gjörloff-Wingren discuss the emerging field of quantitative phase imaging and the novel label-free capabilities that promise to improve and succeed invasive laborious photochemical procedures
Holographic Materials and Optical Systems 2017, 2017 |
Open access book chapter discussing the potential of holographic microscopy in clinical applications
Journal of Medical Imaging, 2017 |
The morphological changes observed occur before and at lower concentrations than a reduction in cell metabolic activity or viability. Three classifiers are compared and we report a best case sensitivity of 88% and specificity of 94% for classification of cells as treated/untreated.
Future science oa, 2015 |
The article provides proof of concept for using holographic microscopy combined with antibody-based microarray technology for detecting morphological changes in captured cells.
PLOS ONE, 2014 |
We show that average cell phase volume results from DHM readings are comparable to the flow cytometry findings. DHM thus provides a non-disruptive alternative to flow cytometry. The technique has the potential to develop into a fast and cost-efficient method for pre-clinical monitoring of cancer treatment efficacy.
- Holography — Basic Principles and Contemporary Applications , 2013 |
- Holography-Research and technologies, 2011 |
- Microscopy: Science, Technology, Applications and Education, 2010 |
Journal of Microscopy, 2008 |
Using a unique non-invasive labelfree cell counting method, results comparable to conventional cell counting using a haemocytometer were produced. The major advantage using HolomonitorTM M2 is the opportunity to easily access information about cell number, size, optical thickness and confluence in an automatic, non-invasive manner.
Faculty of Health and Society,
Jan Waldenströms gata 25, AS:F502
205 06 Malmö, Sweden