Malmö University

PHI and Malmö University have a long-standing collaboration, dating back to 2008. The collaboration has resulted in several peer reviewed publications and a doctoral thesis. In late 2016, the European Commission granted 2.1 million euro to GlycoImaging – a joint cancer research project to develop improved methods for clinically diagnosing cancer.

Current methods for diagnosing cancer primarily focus on the proteins associated with cancer. However, there is increasing evidence that carbohydrates play an important role in the development and pro­gression of malignant cancer. Current methods use and rely on antibodies created by living organisms. These natural antibodies, however, are not sufficiently specific to accurately detect and image carbohydrates.

The GlycoImaging project is coordinated by Malmö University and commercialized by PHI. Additional partners are Bundesanstalt für Materialforschung und Prüfung (Germany’s federal technology research institute), Umeå, Copenhagen and Turku University.

Presentations

Popular lecture on cancer research by Prof. Anette Gjörloff Wingren 2016 (in Swedish).

The GlycoImaging video (in Swedish).

A short presentation of GlycoImaging by Prof. Anette Gjörloff Wingren (in Swedish).

Location

Faculty of Health and Society, 
Malmö University 
Jan Waldenströms gata 25, AS:F502 
205 06 Malmö, Sweden

 

“Oncology research and diagnostics are in need of low-cost and robust probes to detect carbohydrates. The goal of the GlycoImaging project is to meet this need by combining specific carbohydrate probes – in the form of molecular imprinted polymers or ‘plastic antibodies’ – with holographic microscopy.”

Prof. Anette Gjörloff Wingren, Faculty of Health and Society, Malmö University

Peer Reviewed Articles and Book Chapters

  • Cytometry Part A, 2017 | Anette Gjörloff-Wingren

    Prof. Gjörloff-Wingren has used holographic microscopy in cancer related research for over a decade. Gjörloff-Wingren discuss the emerging field of quantitative phase imaging and the novel label-free capabilities that promise to improve and succeed invasive laborious photochemical procedures

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  • Holographic Materials and Optical Systems 2017, 2017 | El-Schich

    Open access book chapter discussing the potential of holographic microscopy in clinical applications

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  • Journal of Medical Imaging, 2017 | Anna Leida Mölder, Johan Persson, Zahra El-Schich, Silvester Czanner, Anette Gjörloff-Wingren

    The morphological changes observed occur before and at lower concentrations than a reduction in cell metabolic activity or viability. Three classifiers are compared and we report a best case sensitivity of 88% and specificity of 94% for classification of cells as treated/untreated.

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  • Future science oa, 2015 | Zahra El-Schich, Emmy Nilsson, Anna S Gerdtsson, Christer Wingren and Anette Gjörloff Wingren

    The article provides proof of concept for using holographic microscopy combined with antibody-based microarray technology for detecting morphological changes in captured cells.

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  • PLOS ONE, 2014 | Maria Falck Miniotis, Anthonny Mukwaya and Anette Gjörloff Wingren

    We show that average cell phase volume results from DHM readings are comparable to the flow cytometry findings. DHM thus provides a non-disruptive alternative to flow cytometry. The technique has the potential to develop into a fast and cost-efficient method for pre-clinical monitoring of cancer treatment efficacy.

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  • Holography — Basic Principles and Contemporary Applications , 2013 | K. Alm, Z. El-Schich, M. Falck Miniotis, A. Gjörloff Wingren, B. Janicke and S. Oredsson
  • Holography-Research and technologies, 2011 | K. Alm, H. Cirenajwis, L. Gisselsson, A. Gjörloff Wingren, B. Janicke, A. Mölder, S. Oredsson, J. Persson
  • Microscopy: Science, Technology, Applications and Education, 2010 | Z. El-Schish, A. Mölder, M. Sebesta, L. Gisselsson, K. Alm, A. Gjörloff Wingren
  • Journal of Microscopy, 2008 | Anna Mölder et al

    Using a unique non-invasive labelfree cell counting method, results comparable to conventional cell counting using a haemocytometer were produced. The major advantage using HolomonitorTM M2 is the opportunity to easily access information about cell number, size, optical thickness and confluence in an automatic, non-invasive manner.

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Docteral Theses

  • Zahra El-Schich, 2016 | Health and Society, Malmö University
    To analyze morphological changes of death cells, HoloMonitor was used. Digital holographic microscopy is an approach for label-free non-invasive 3D imaging of cultured cells. We have analyzed cell death of adherent cancer cells using HoloMonitor and developed it to analyze suspension cells by combining this technique with antibody based micro­assays. HoloMonitor can be used for cell-death induced cell analysis of both adherent cells and suspension cells.
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Featured Applications

Label-free Cell Cycle Analysis, by Malmö University

Live cell imaging cytometry of antibody captured suspension cells, by Malmö University
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